Biodegradation of sulfadiazine in anaerobic co-digestion of swine manure and food waste

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology Pub Date : 2025-04-11 DOI:10.1016/j.biortech.2025.132518

Jialin Liang , Yu Zhang , Jiaqi Zhang , Zhizhuang Xie , Huiyi Chen , Konrad Koch , Aibin Hu , Liwen Luo

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Abstract

This study explored the feasibility of employing the AcoD process for the removal of antibiotics and examined the impact of antibiotics on system performance. Sulfadiazine (SDZ), a prevalent broad-spectrum sulfonamide antibiotic in veterinary medicine, was selected as the model compound. Results showed that with the presence of SDZ at a concentration of 450 mg/kg total solids, the cumulative methane yield demonstrated a substantial decline of 79.2 % compared to the control group. The specific removal rate of SDZ was 47.5 % at 450 mg SDZ/kg total solids, surpassing those observed in traditional mono-anaerobic treatment processes. The elimination of SDZ by the AcoD system was predominantly ascribed to biodegradation. Within the AcoD system, cytochrome P450 enzyme (CYP450) served as the crucial enzyme in the biodegradation of SDZ. From a molecular point of view, the main interaction sites of SDZ with CYP450 enzyme were located as Thr258, Glu257, Pro428, Ala254, and Val318. Six transformation products were identified in the biodegradation process. Community diversity revealed that the predominant genera, Syntrophomonadaceae, Acinetobacter, AUTHM297, and Anaerolineaceae, were enriched in the AcoD process, which probably contributed to SDZ removal. In summary, the AcoD system may possess sufficient robustness to transform SDZ antibiotic.

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猪粪与食物垃圾厌氧共消化中磺胺嘧啶的生物降解

本研究探讨了采用AcoD工艺去除抗生素的可行性，并考察了抗生素对系统性能的影响。以兽药中常用的广谱磺胺类抗生素磺胺嘧啶（SDZ）为模型化合物。结果表明，当SDZ浓度为450 mg/kg时，累积甲烷产率较对照组显著下降79.2%。在450 mg SDZ/kg总固体时，SDZ的比去除率为47.5%，优于传统的单厌氧处理工艺。AcoD系统对SDZ的消除主要归因于生物降解。在AcoD系统中，细胞色素P450酶（CYP450）是SDZ生物降解的关键酶。从分子角度看，SDZ与CYP450酶的主要相互作用位点为Thr258、Glu257、Pro428、Ala254和Val318。在生物降解过程中鉴定出6种转化产物。群落多样性表明，AcoD过程富集了优势属Syntrophomonadaceae、Acinetobacter、AUTHM297和Anaerolineaceae，这可能有助于SDZ的去除。综上所述，AcoD系统可能具有足够的鲁棒性来转化SDZ抗生素。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioresource Technology 工程技术-能源与燃料

CiteScore

20.80

自引率

19.30%

发文量

2013

审稿时长

12 days

期刊介绍： Bioresource Technology publishes original articles, review articles, case studies, and short communications covering the fundamentals, applications, and management of bioresource technology. The journal seeks to advance and disseminate knowledge across various areas related to biomass, biological waste treatment, bioenergy, biotransformations, bioresource systems analysis, and associated conversion or production technologies. Topics include: • Biofuels: liquid and gaseous biofuels production, modeling and economics • Bioprocesses and bioproducts: biocatalysis and fermentations • Biomass and feedstocks utilization: bioconversion of agro-industrial residues • Environmental protection: biological waste treatment • Thermochemical conversion of biomass: combustion, pyrolysis, gasification, catalysis.