Establishment of a microfluidic and RPA-based platform for rapid multi-sample detection of Mycoplasma pneumoniae

IF 2.1 4区医学 Q3 INFECTIOUS DISEASES

Diagnostic microbiology and infectious disease Pub Date : 2025-04-10 DOI:10.1016/j.diagmicrobio.2025.116840

Ling Zhang , Cheng-Di Sun , Jiao Luo , Li-Guo Liang , Si-Ming Lu

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引用次数: 0

Abstract

Background

Mycoplasma pneumoniae (M. pneumoniae) is a major respiratory pathogen causing atypical community-acquired pneumonia. Early and rapid diagnosis and timely detection of pathogens are crucial. RPA is an emerging PCR technology that allows amplification at room temperature compared to conventional PCR technology, while combining the advantages of rapid detection and high specificity of PCR technology, enabling rapid and accurate detection of pathogens.

Methods and results

In this study, we established a platform for detection of M. pneumoniae based on microfluidic and RPA technologies. The RPA technology is performed in 15 to 20 minutes, and only M. pneumoniae is significantly amplified at a sensitivity of 10 copies/μL; microfluidic technology provides lean instrumentation and a convenient interactive system. Three groups negative and positive QCs and 18 clinical samples can be tested in a single run.

Significance

In conclusion, we have established a microfluidic molecular assay operating system based on RPA technology, with the advantages of being simple, rapid, accurate and economical, and providing a new tool for laboratory detection of M. pneumoniae.

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来源期刊

Diagnostic microbiology and infectious disease 医学-传染病学

CiteScore

5.30

自引率

3.40%

发文量

149

审稿时长

56 days

期刊介绍： Diagnostic Microbiology and Infectious Disease keeps you informed of the latest developments in clinical microbiology and the diagnosis and treatment of infectious diseases. Packed with rigorously peer-reviewed articles and studies in bacteriology, immunology, immunoserology, infectious diseases, mycology, parasitology, and virology, the journal examines new procedures, unusual cases, controversial issues, and important new literature. Diagnostic Microbiology and Infectious Disease distinguished independent editorial board, consisting of experts from many medical specialties, ensures you extensive and authoritative coverage.