Divergent mitigation mechanisms of soil antibiotic resistance genes by biochar from different agricultural wastes

IF 7.6 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Environmental Pollution Pub Date : 2025-04-12 DOI:10.1016/j.envpol.2025.126247

Yu Yao , Peiyang Yang , Bin Wang , Qiao Xu , Fanhao Song , Yabo Wang , Yingdong Zhao , Lei Guo , Xiaozhi Wang , Meiling Xu

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Abstract

Biochar, produced from agricultural waste, provides a sustainable solution for effective waste management and soil remediation. The potential and mechanisms of biochar derived from primary agricultural sources (plant residues and animal manure) to mitigate the antibiotic resistance genes (ARGs) pollution in agricultural soil remain unexplored. To address this, a 60-day microcosm experiment was conducted, applying biochar derived from either corn straw (B1) or chicken manure (B2) to sulfamethazine and tetracycline-contaminated soil. The results demonstrated that B1 had richer functional groups than B2. B1 increased soil pH (+1.63 %) and total carbon (+24.56 %), but it decreased the abundance of norank_Vicinamibacteraceae (−35.71 %) and Haliangium (−42.11 %), and inhibited the dissemination of tetM and tetW by 57.76 % and 39.17 %, respectively. Comparatively, B2 significantly increased soil dissolved organic carbon (+161.66 %) and decreased the abundance of potential ARGs hosts (Acidibacter, −40.32 %), leading to reduced sul2 abundance (−33.47 %). Besides, B2 enhanced soil total nitrogen and bacterial diversity, and further reduced tetW abundance (−33.76 %). Overall, this study revealed divergent mechanisms and potential for mitigating soil ARGs transmission by biochar derived from corn straw and chicken manure. This study contributes to developing more effective strategies for managing ARGs in contaminated soil and mitigating their environmental risks.

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不同农业废弃物生物炭对土壤抗生素抗性基因的不同缓解机制

利用农业废弃物生产的生物炭为有效的废弃物管理和土壤修复提供了一种可持续的解决方案。从初级农业资源（植物残留物和动物粪便）中提取的生物炭在减轻农业土壤中抗生素抗性基因（ARGs）污染方面的潜力和机制仍未得到充分探索。为了解决这个问题，我们进行了一项为期 60 天的小宇宙实验，将从玉米秸秆（B1）或鸡粪（B2）中提取的生物炭施用到磺胺甲基嘧啶和四环素污染的土壤中。结果表明，B1 比 B2 具有更丰富的功能基团。B1 提高了土壤的 pH 值（+1.63%）和总碳（+24.56%），但降低了诺安克霉素菌科（-35.71%）和卤虫菌科（-42.11%）的丰度，并抑制了 tetM 和 tetW 的传播，抑制率分别为 57.76% 和 39.17%。相比之下，B2 显著增加了土壤溶解有机碳（+161.66%），降低了潜在 ARGs 宿主（酸杆菌，-40.32%）的丰度，导致 sul2 丰度降低（-33.47%）。此外，B2 提高了土壤全氮和细菌多样性，进一步降低了 tetW 丰度（-33.76%）。总之，这项研究揭示了玉米秸秆和鸡粪提取的生物炭减轻土壤中 ARGs 传播的不同机制和潜力。这项研究有助于制定更有效的策略来管理受污染土壤中的 ARGs 并降低其环境风险。

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来源期刊

Environmental Pollution 环境科学-环境科学

CiteScore

16.00

自引率

6.70%

发文量

2082

审稿时长

2.9 months

期刊介绍： Environmental Pollution is an international peer-reviewed journal that publishes high-quality research papers and review articles covering all aspects of environmental pollution and its impacts on ecosystems and human health. Subject areas include, but are not limited to: • Sources and occurrences of pollutants that are clearly defined and measured in environmental compartments, food and food-related items, and human bodies; • Interlinks between contaminant exposure and biological, ecological, and human health effects, including those of climate change; • Contaminants of emerging concerns (including but not limited to antibiotic resistant microorganisms or genes, microplastics/nanoplastics, electronic wastes, light, and noise) and/or their biological, ecological, or human health effects; • Laboratory and field studies on the remediation/mitigation of environmental pollution via new techniques and with clear links to biological, ecological, or human health effects; • Modeling of pollution processes, patterns, or trends that is of clear environmental and/or human health interest; • New techniques that measure and examine environmental occurrences, transport, behavior, and effects of pollutants within the environment or the laboratory, provided that they can be clearly used to address problems within regional or global environmental compartments.