Colorimetric and smartphone-based dual modality sensing platform for detection of ascorbic acid

IF 4.1 3区工程技术 Q2 ENGINEERING, ELECTRICAL & ELECTRONIC

Sensors and Actuators A-physical Pub Date : 2025-04-10 DOI:10.1016/j.sna.2025.116573

Ekta Arjundas Kukreja , Ravi Mani Tripathi , Sang J. Chung

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引用次数: 0

Abstract

Ascorbic acid (AA) is a vital vitamin possessing antioxidant properties which is used to prevent and treat diseases like scurvy and cancer. Therefore, precise detection of ultra-low concentrations of AA is one of the crucial challenges and is also important for therapeutic applications. We have evaluated for the first time the nanozymatic activity of ZnO-Pd nanobolts for the detection of AA. The present study has developed a nanozyme-based colorimetric detection assay for AA using a chromogenic substrate 3,3′,5,5′-Tetramethylbenzidine (TMB). ZnO-Pd nanobolts exhibited strong peroxidase mimetic activity by oxidizing chromogenic substrate TMB. Colorless TMB was oxidised to blue color ox-TMB by the action of ZnO-Pd nanobolts in the presence of hydrogen peroxide (H₂O₂). The detection of AA was based on a turn-off approach where addition of AA to the reaction mixture induced a reduction reaction. This reduces the ox-TMB to TMB causing the blue color of the solution to fade. The assay was tested with multiple serum containing substances and showed high specificity toward AA. The sensitivity of the assay was studied over a linear range of 0.05–128 µM, and the results were analysed by measuring the intensity of the colored solution using UV-Vis spectroscopy. The LOD and LOQ of the assay were found to be 0.031 µM and 0.094 µM respectively. Additionally, smartphone analysis was carried out for attaining a relationship between the color intensity of the reaction solution and concentrations of the AA. The method was used for determination of AA in vitamin C tablets for assessing the practical application of the assay.

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基于比色法和智能手机的抗坏血酸检测双模式传感平台

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来源期刊

Sensors and Actuators A-physical 工程技术-工程：电子与电气

CiteScore

8.10

自引率

6.50%

发文量

630

审稿时长

49 days

期刊介绍： Sensors and Actuators A: Physical brings together multidisciplinary interests in one journal entirely devoted to disseminating information on all aspects of research and development of solid-state devices for transducing physical signals. Sensors and Actuators A: Physical regularly publishes original papers, letters to the Editors and from time to time invited review articles within the following device areas: • Fundamentals and Physics, such as: classification of effects, physical effects, measurement theory, modelling of sensors, measurement standards, measurement errors, units and constants, time and frequency measurement. Modeling papers should bring new modeling techniques to the field and be supported by experimental results. • Materials and their Processing, such as: piezoelectric materials, polymers, metal oxides, III-V and II-VI semiconductors, thick and thin films, optical glass fibres, amorphous, polycrystalline and monocrystalline silicon. • Optoelectronic sensors, such as: photovoltaic diodes, photoconductors, photodiodes, phototransistors, positron-sensitive photodetectors, optoisolators, photodiode arrays, charge-coupled devices, light-emitting diodes, injection lasers and liquid-crystal displays. • Mechanical sensors, such as: metallic, thin-film and semiconductor strain gauges, diffused silicon pressure sensors, silicon accelerometers, solid-state displacement transducers, piezo junction devices, piezoelectric field-effect transducers (PiFETs), tunnel-diode strain sensors, surface acoustic wave devices, silicon micromechanical switches, solid-state flow meters and electronic flow controllers. Etc...