Etiología de la queratitis infecciosa

Q3 Medicine

Archivos De La Sociedad Espanola De Oftalmologia Pub Date : 2025-04-01 DOI:10.1016/j.oftal.2024.11.003

C. Piña-Delgado , M. Bolaños-Rivero , I.I. Guedes Guedes , L. Siguero-Martín , I. de Miguel-Martínez

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引用次数: 0

Abstract

Objetives

Analyze the etiology of infectious keratitis in our hospital.

Material and methods

Retrospective study in which the medical records of patients were reviewed in which a keratitis-producing microorganism was detected during the last 9 years (January 2014-December 2022). The sample was obtained by corneal scraping and seeded in non-selective media. Bacterial and fungal identification was carried out by mass spectrometry and viral identification by polymerase chain reaction. Sensitivity was obtained using disk-plate antibiograms, Etest or broth microdilution systems.

Results

A total of 433 samples of corneal scrapings belonging to 416 patients were processed. Of the total samples, 196 were positive (44,3%). The average age was 55 years, with 51% being women. Regarding the etiology, we found the following isolates: Gram-positive bacteria (N = 83; 44%), highlighting Staphylococcus aureus (N = 33), coagulase-negative staphylococci (N = 26), being Staphylococcus epidermidis the most frequent (N = 19); gram-negative bacteria (N = 67; 35%), including: Pseudomonas aeruginosa (N = 31), 42% associated with the use of contact lenses and enterobacterales (N = 16); anaerobes (N = 19), which 18 isolates were Cutibacterium acnes. Regarding viral etiology (N = 10): herpes simple type 1 (N = 7) and varicella-zoster virus (N = 3). Finally, the fungal etiology (N = 13), highlighting Candida spp. (N = 10).

Conclusions

The main agents of infectious keratitis are Staphylococcus aureus and Pseudomonas aeruginosa. The causative agent was detected in 44,3% of the samples, so microbiological analysis of these samples is highly advisable.

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传染性角膜炎的病因

目的分析我院感染性角膜炎的病因。材料与方法回顾性分析近9年（2014年1月- 2022年12月）检测到一种角膜炎产生微生物的患者病历。样品通过角膜刮痧获得，并在非选择性培养基中播种。细菌和真菌鉴定采用质谱法，病毒鉴定采用聚合酶链反应法。使用盘片抗生素谱、est或肉汤微量稀释系统获得敏感性。结果共处理416例患者角膜刮擦标本433份。阳性196份（44.3%）。平均年龄为55岁，其中51%为女性。在病原学方面，我们发现以下分离株：革兰氏阳性菌(N = 83；44%)，其中以金黄色葡萄球菌（N = 33）、凝固酶阴性葡萄球菌（N = 26）为主，以表皮葡萄球菌最多（N = 19）；革兰氏阴性菌(N = 67；35%)，包括：铜绿假单胞菌（N = 31）， 42%与使用隐形眼镜和肠杆菌（N = 16）有关；厌氧菌（N = 19），其中18株为痤疮表皮杆菌。病毒病原学（N = 10）：单纯疱疹1型（N = 7）和水痘带状疱疹病毒（N = 3）。真菌病原学（N = 13），突出念珠菌（N = 10）。结论感染性角膜炎的主要病原体是金黄色葡萄球菌和铜绿假单胞菌。在44.3%的样品中检测到病原体，因此对这些样品进行微生物学分析是非常可取的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Archivos De La Sociedad Espanola De Oftalmologia Medicine-Ophthalmology

CiteScore

1.20

自引率

0.00%

发文量

109

审稿时长

78 days

期刊介绍： La revista Archivos de la Sociedad Española de Oftalmología, editada mensualmente por la propia Sociedad, tiene como objetivo publicar trabajos de investigación básica y clínica como artículos originales; casos clínicos, innovaciones técnicas y correlaciones clinicopatológicas en forma de comunicaciones cortas; editoriales; revisiones; cartas al editor; comentarios de libros; información de eventos; noticias personales y anuncios comerciales, así como trabajos de temas históricos y motivos inconográficos relacionados con la Oftalmología. El título abreviado es Arch Soc Esp Oftalmol, y debe ser utilizado en bibliografías, notas a pie de página y referencias bibliográficas.