Biocascade-inspired amplified oxygen vacancy effect on facet-engineered BiOI for ultrasensitive photoelectrochemical detection of 8-oxoguanine DNA glycosylase

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics Pub Date : 2025-04-09 DOI:10.1016/j.bios.2025.117466

Mengmeng Gu, Xia Yu, Qing Zhou, Xiuming Wu, Jing Wang, Guang-Li Wang

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引用次数: 0

Abstract

Sensitive detection of 8-oxoguanine DNA glycosylase 1 (hOGG1) activity is essential for early cancer screening and therapy, yet the potential of photoelectrochemistry (PEC) for hOGG1 detection is untapped. Herein, we explore a new bioreaction for sensitive PEC detection of hOGG1 through biocascade reaction provoked amplified oxygen vacancy (O_V) effect on facet-engineered BiOI. Specifically, the recognition of hOGG1 activated the catalytic peptide hydrolysis reaction of thrombin (Thr), producing the O_V stimulator p-aminophenol (AP). AP was recycled via the diphosphatase (DI, EC 1.6.99.2) mediated reaction, inducing the formation of abundant surface O_V on BiOI with exposed (110) facet (BI-110). This process strikingly enhanced the carrier separation efficiency and augmented the photocurrent gain, enabling highly sensitive detection of hOGG1 with a linear range of 1.0 × 10⁻⁴ to 80 U/mL and a low detection limit of 2.0 × 10⁻⁵ U/mL. This study addresses the challenge of developing effective PEC assays for hOGG1 by elucidating a new principle of the biocascade reaction-sparked O_V effect with facet selectivity, thus filling a gap in PEC detection method for this enzyme.

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生物级联激发的放大氧空位效应在面工程BiOI上用于超灵敏光电化学检测8-氧鸟嘌呤DNA糖基化酶

8-氧鸟嘌呤DNA糖基酶1 （hOGG1）活性的灵敏检测对于早期癌症筛查和治疗至关重要，但光电化学（PEC）检测hOGG1的潜力尚未开发。在此，我们探索了一种新的生物反应，通过生物级联反应引发放大氧空位（OV）效应，对面工程BiOI进行灵敏的PEC检测。具体来说，hOGG1的识别激活了凝血酶（Thr）的催化肽水解反应，产生OV刺激物对氨基酚（AP）。AP通过二磷酸酶（DI, EC 1.6.99.2）介导的反应再循环，诱导暴露（110）facet （BI-110）的BiOI表面形成丰富的OV。该工艺显著提高了载流子分离效率，增加了光电流增益，实现了对hOGG1的高灵敏度检测，线性范围为1.0 × 10−4 ~ 80 U/mL，检测限低至2.0 × 10−5 U/mL。本研究通过阐明具有小面选择性的生物级联反应引发的OV效应的新原理，解决了开发有效的hOGG1 PEC检测方法的挑战，从而填补了该酶PEC检测方法的空白。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biosensors and Bioelectronics 工程技术-电化学

CiteScore

20.80

自引率

7.10%

发文量

1006

审稿时长

29 days

期刊介绍： Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.