The function of BoVDAC3 from broccoli in oxidative stress response and programmed cell death in BY-2 cells

IF 6.4 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology Pub Date : 2025-04-11 DOI:10.1016/j.postharvbio.2025.113582

Yuan Qian , Jiahui Chen , Shifeng Cao , Min Luo , Wenhui Jiao , Yi Chen , Yingying Wei , Xingfeng Shao , Feng Xu

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引用次数: 0

Abstract

Voltage dependent anion channel protein (VDAC) had been shown to played a critical role in programmed cell death (PCD). However, the functional role of BoVDAC3 in oxidative stress response and PCD process of postharvest broccoli remains unclear. In this study, BoVDAC3 was overexpressed in tobacco bright yellow-2 (BY-2) cells to investigate its function. The results showed that the cell morphology and nuclear integrity of transgenic BY-2 cells were severely disrupted after oxidative stress compared with wild-type (WT) cells. BoVDAC3 overexpressing dreadfully increased the content of reactive oxygen species (ROS) and malondialdehyde (MDA) in BY-2 cells, while simultaneously decreasing proline (Pro) synthesis and impairing the function of the AsA-GSH cycle, along with key antioxidant enzymes. Moreover, the expression levels of AsA-GSH cycle-related genes (NtGST, NtAO, NtAPX), Pro synthesis-related genes (NtP5CR, NtP5CS, Ntδ-OAT), antioxidant enzyme genes (NtSOD, NtPOD, NtCAT), and the anti-apoptotic gene (NtDAD1) were markedly reduced in the transgenic cells compared to the control group following H₂O₂ treatment. Conversely, the expression levels of apoptotic genes (NtSIPK, NtERF3) were considerably elevated under the same experimental conditions. Furthermore, treatment with 4,4′-diisothiocyanostilbene-2,2′-disulfonic acid (DIDS), a VDAC inhibitor, produced the opposite phenotype to BoVDAC3-overexpressing cells. These findings suggest that BoVDAC3 overexpression may accelerate ROS accumulation, thereby inducing PCD in BY-2 cells.

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来源期刊

Postharvest Biology and Technology 农林科学-农艺学

CiteScore

12.00

自引率

11.40%

发文量

309

审稿时长

38 days

期刊介绍： The journal is devoted exclusively to the publication of original papers, review articles and frontiers articles on biological and technological postharvest research. This includes the areas of postharvest storage, treatments and underpinning mechanisms, quality evaluation, packaging, handling and distribution of fresh horticultural crops including fruit, vegetables, flowers and nuts, but excluding grains, seeds and forages. Papers reporting novel insights from fundamental and interdisciplinary research will be particularly encouraged. These disciplines include systems biology, bioinformatics, entomology, plant physiology, plant pathology, (bio)chemistry, engineering, modelling, and technologies for nondestructive testing. Manuscripts on fresh food crops that will be further processed after postharvest storage, or on food processes beyond refrigeration, packaging and minimal processing will not be considered.