Exploring the Material Basis of Taxillus chinensis in the Treatment of Hyperuricemia Nephropathy Through Absorbed Into Blood Component Analysis and Network Pharmacology

IF 1.8 4区 医学 Q4 BIOCHEMICAL RESEARCH METHODS
Jiemei Liang, Hua Zhu, Qiyuan Yang, Zhouwei Li, Xiang Meng, Lanlan Fan, Li Li
{"title":"Exploring the Material Basis of Taxillus chinensis in the Treatment of Hyperuricemia Nephropathy Through Absorbed Into Blood Component Analysis and Network Pharmacology","authors":"Jiemei Liang,&nbsp;Hua Zhu,&nbsp;Qiyuan Yang,&nbsp;Zhouwei Li,&nbsp;Xiang Meng,&nbsp;Lanlan Fan,&nbsp;Li Li","doi":"10.1002/bmc.70066","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p><i>Taxillus chinensis</i> (DC) Danser (<i>T. chinensis</i>) is broadly used in traditional Chinese medicine. Although pharmacological research shows that the ethyl acetate extract of <i>T. chinensis</i> (JEA) has beneficial effects in treating hyperuricemic nephropathy (HN), the active components and potential mechanisms for these effects remain unclear. This study aims to predict the effective components and mechanism of JEA for HN. Firstly, we adopted UHPLC-Q-Exactive HFXMS technology to analyze the chemical profile of JEA and the absorbed prototype ingredients in rat plasma. In addition, network pharmacology methods were utilized by us to elaborate on the active compounds, signaling pathways, and potential mechanisms of JEA in treating HN. Finally, a UPLC method was established to screen potential chemicals that can effectively inhibit the activity of xanthine oxidase (XOD). A total of 92 components were systematically characterized in JEA. Of those, 46 compounds were identified in the plasma of rats administered with JEA extract. Through network pharmacology, 10 potential active components, 10 crucial target genes, and 20 pathways were predicted to be involved in the JEA-mediated treatment of HN. The molecular docking results indicated that oxyresveratrol, isorhammeiol, and robinetwere exhibited strong binding affinities for GAPDH, PPARG, and ALB. The analysis of the XOD inhibition experiment suggested that dihydromyricetin and oxyresveratrol exhibited potent inhibitory effects on XOD, with IC<sub>50</sub> values of 0.48 and 0.68 mg·mL<sup>−1</sup>. This study preliminarily identified the potential effective components of JEA and revealed its underlying molecular mechanisms of action in preventing HN, which will improve our further studies on JEA to treat HN.</p>\n </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 5","pages":""},"PeriodicalIF":1.8000,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedical Chromatography","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/bmc.70066","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0

Abstract

Taxillus chinensis (DC) Danser (T. chinensis) is broadly used in traditional Chinese medicine. Although pharmacological research shows that the ethyl acetate extract of T. chinensis (JEA) has beneficial effects in treating hyperuricemic nephropathy (HN), the active components and potential mechanisms for these effects remain unclear. This study aims to predict the effective components and mechanism of JEA for HN. Firstly, we adopted UHPLC-Q-Exactive HFXMS technology to analyze the chemical profile of JEA and the absorbed prototype ingredients in rat plasma. In addition, network pharmacology methods were utilized by us to elaborate on the active compounds, signaling pathways, and potential mechanisms of JEA in treating HN. Finally, a UPLC method was established to screen potential chemicals that can effectively inhibit the activity of xanthine oxidase (XOD). A total of 92 components were systematically characterized in JEA. Of those, 46 compounds were identified in the plasma of rats administered with JEA extract. Through network pharmacology, 10 potential active components, 10 crucial target genes, and 20 pathways were predicted to be involved in the JEA-mediated treatment of HN. The molecular docking results indicated that oxyresveratrol, isorhammeiol, and robinetwere exhibited strong binding affinities for GAPDH, PPARG, and ALB. The analysis of the XOD inhibition experiment suggested that dihydromyricetin and oxyresveratrol exhibited potent inhibitory effects on XOD, with IC50 values of 0.48 and 0.68 mg·mL−1. This study preliminarily identified the potential effective components of JEA and revealed its underlying molecular mechanisms of action in preventing HN, which will improve our further studies on JEA to treat HN.

通过血液成分分析及网络药理学探讨红豆杉治疗高尿酸血症肾病的物质基础
紫杉(Taxillus chinensis, DC)是一种应用广泛的中药。虽然药理学研究表明,三叶草乙酸乙酯提取物(JEA)对治疗高尿酸血症肾病(HN)有有益作用,但其有效成分和潜在机制尚不清楚。本研究旨在预测JEA治疗HN的有效成分及作用机制。首先,我们采用UHPLC-Q-Exactive HFXMS技术分析了JEA和大鼠血浆中吸收的原型成分的化学特征。此外,我们还利用网络药理学方法详细阐述了JEA治疗HN的活性化合物、信号通路和潜在机制。最后,建立了UPLC筛选黄嘌呤氧化酶(xanthine oxidase, XOD)活性抑制物质的方法。在JEA中对92个组分进行了系统表征。其中,在给予JEA提取物的大鼠血浆中鉴定出46种化合物。通过网络药理学,预测了10种潜在活性成分、10种关键靶基因和20种途径参与jea介导的HN治疗。分子对接结果表明,氧化白藜芦醇、异鼠醇和robinet对GAPDH、ppar和ALB具有较强的结合亲和力。抑制XOD实验分析表明,二氢杨梅素和氧化白藜芦醇对XOD具有较强的抑制作用,IC50值分别为0.48和0.68 mg·mL−1。本研究初步鉴定了JEA的潜在有效成分,揭示了JEA预防HN的潜在分子机制,为进一步开展JEA治疗HN的研究提供基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Biomedical Chromatography
Biomedical Chromatography 生物-分析化学
CiteScore
3.60
自引率
5.60%
发文量
268
审稿时长
2.3 months
期刊介绍: Biomedical Chromatography is devoted to the publication of original papers on the applications of chromatography and allied techniques in the biological and medical sciences. Research papers and review articles cover the methods and techniques relevant to the separation, identification and determination of substances in biochemistry, biotechnology, molecular biology, cell biology, clinical chemistry, pharmacology and related disciplines. These include the analysis of body fluids, cells and tissues, purification of biologically important compounds, pharmaco-kinetics and sequencing methods using HPLC, GC, HPLC-MS, TLC, paper chromatography, affinity chromatography, gel filtration, electrophoresis and related techniques.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信