Enhancing Copper (Cu) Phytostabilization Efficiency of Commelina communis by Inoculating with Endophytic Bacillus sp. D2: Impacts on Plant Growth, Soil Ecological Characteristics, and Underground Bacterial Community

IF 3.8 4区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Water, Air, & Soil Pollution Pub Date : 2025-04-11 DOI:10.1007/s11270-025-07973-5

Jun Fu, Maodi Ran, Han Zhou, Ying Jiao, Ying Shi, Jiaokun Li

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引用次数: 0

Abstract

Phytoremediation assisted by endophytic bacteria is a promising strategy to enhance the remediation efficiency of heavy metals in contaminated soil. In this study, the capacity and role of the endophytic Bacillus sp. D2, previously isolated from Commelina communis growing near a copper (Cu) mine, in assisting the phytoremediation were evaluated. Results showed that inoculation of Bacillus sp. D2 significantly enhanced the biomass production of C. communis by 131.06% under high level of Cu stress. Additionally, the oxidative damages caused by Cu toxicity in C. communis tissues were alleviated as evidenced by significant reductions in malondialdehyde (MDA), superoxide anion (O₂^•−) and proline content following Bacillus sp. D2 inoculation. Meanwhile, the activities of antioxidant enzymes in plant leaves presented upward trends after Bacillus sp. D2 inoculation. Notably, Bacillus sp. D2 inoculation significantly decreased Cu uptake and translocation by C. communis, while enhancing the Cu stabilization in contaminated soils. Furthermore, soil enzyme activities (acid phosphatase, catalase, and urease), as well as the richness of soil bacterial communities in Cu-contaminated soil increased following Bacillus sp. D2 inoculation. Importantly, the inoculation specifically augmented the relative abundance of key bacterial taxa (including Pseudomonas and Sphingomonadaceae) in the rhizosphere soil, which was positively correlated with soil nutrients cycling and plant growth. Our findings suggest that the endophytic strain Bacillus sp. D2 can strengthen the phytostabilization efficiency of Cu by C. communis through its beneficial effects on plant physio-biochemistry, soil quality and bacterial microecology, which provides a basis for the relative application to Cu-contaminated soils.

Graphical Abstract

查看原文本刊更多论文

通过接种内生芽孢杆菌 D2 提高共生草本植物的铜（Cu）植物稳定效率：对植物生长、土壤生态学特征和地下细菌群落的影响

内生细菌辅助植物修复是提高污染土壤重金属修复效率的有效途径。本研究对生长在铜矿附近的Commelina communis中分离到的内生芽孢杆菌D2在植物修复中的能力和作用进行了评价。结果表明，在高Cu胁迫下，接种芽孢杆菌D2显著提高了c.s communis的生物量，提高了131.06%。此外，接种芽孢杆菌D2后，丙二醛（MDA）、超氧阴离子（O2•−）和脯氨酸含量显著降低，减轻了铜毒性对芦笋组织的氧化损伤。同时，接种芽孢杆菌D2后，植物叶片中抗氧化酶活性呈上升趋势。接种芽孢杆菌D2显著降低了铜的吸收和转运，增强了铜在污染土壤中的稳定性。此外，接种芽孢杆菌D2后，cu污染土壤的酸性磷酸酶、过氧化氢酶和脲酶活性以及细菌群落的丰富度均有所增加。重要的是，接种特异性地增加了根际土壤中关键细菌类群（包括假单胞菌科和鞘单胞菌科）的相对丰度，这与土壤养分循环和植物生长呈正相关。研究结果表明，内生菌株芽孢杆菌D2可以通过对植物生理生化、土壤质量和细菌微生态等方面的有益作用来增强铜的植物稳定效率，为在铜污染土壤上的相对应用提供了依据。图形抽象

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来源期刊

Water, Air, & Soil Pollution 环境科学-环境科学

CiteScore

4.50

自引率

6.90%

发文量

448

审稿时长

2.6 months

期刊介绍： Water, Air, & Soil Pollution is an international, interdisciplinary journal on all aspects of pollution and solutions to pollution in the biosphere. This includes chemical, physical and biological processes affecting flora, fauna, water, air and soil in relation to environmental pollution. Because of its scope, the subject areas are diverse and include all aspects of pollution sources, transport, deposition, accumulation, acid precipitation, atmospheric pollution, metals, aquatic pollution including marine pollution and ground water, waste water, pesticides, soil pollution, sewage, sediment pollution, forestry pollution, effects of pollutants on humans, vegetation, fish, aquatic species, micro-organisms, and animals, environmental and molecular toxicology applied to pollution research, biosensors, global and climate change, ecological implications of pollution and pollution models. Water, Air, & Soil Pollution also publishes manuscripts on novel methods used in the study of environmental pollutants, environmental toxicology, environmental biology, novel environmental engineering related to pollution, biodiversity as influenced by pollution, novel environmental biotechnology as applied to pollution (e.g. bioremediation), environmental modelling and biorestoration of polluted environments. Articles should not be submitted that are of local interest only and do not advance international knowledge in environmental pollution and solutions to pollution. Articles that simply replicate known knowledge or techniques while researching a local pollution problem will normally be rejected without review. Submitted articles must have up-to-date references, employ the correct experimental replication and statistical analysis, where needed and contain a significant contribution to new knowledge. The publishing and editorial team sincerely appreciate your cooperation. Water, Air, & Soil Pollution publishes research papers; review articles; mini-reviews; and book reviews.