In vitro antimicrobial activity of Amburana cearensis resin against Staphylococcus aureus isolated from caprine mastitis

The Microbe Pub Date : 2025-04-10 DOI:10.1016/j.microb.2025.100335

Dailli Ingrid de Brito Lima , Nayara Andreo , Priscila Izidro de Figueirêdo , José Gledyson da Silva , Mariana de Barros , Junnia Luísa Pena , Renata de Faria Silva Souza , Tania Maria Sarmento da Silva , Maria Aparecida Scatamburlo Moreira , Mateus Matiuzzi da Costa

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Abstract

The aim with the study was to evaluate the in vitro potential of Amburana cearensis resin for the treatment of caprine mastitis caused by Staphylococcus aureus. It was tested the resin antimicrobial activity, anti-biofilm and synergism. Bovine mammary epithelial cells (MAC-T) were used to evaluate the resin’s interference with the adhesion and invasion of the isolates and the resin cytotoxic effect. The resin showed antibacterial activity (195.3 μg/mL to 1562.5 μg mL), interfering with biofilm formation and with the mature structure. Synergism of the resin with oxacillin in the MRSA (methicillin resistant) isolate reduced the bactericidal concentration of the antibiotic. Significant reductions (p < 0.01) in adhesion and invasion of the isolates were observed. Also, there was a small reduction in cell viability, with 195.3 μg / mL representing the best minimum inhibitory concentration of the resin, reducing only 1.4 % of MAC-T viability. The results showed that the A. cearensis resin represents a possible alternative for use as an antimicrobial herbal medicine. A. cearensis resin highlights the potential of natural products in veterinary infections, which can contribute to reducing selection pressure in bacterial pathogens and also directly impact human health.

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羊乳草树脂对山羊乳腺炎金黄色葡萄球菌的体外抑菌活性研究

本研究的目的是评价羊绒树脂在体外治疗由金黄色葡萄球菌引起的绵羊乳腺炎的潜力。对树脂进行了抗菌活性、抗生物膜活性和增效性试验。用牛乳腺上皮细胞（MAC-T）评价了树脂对分离物粘附和侵袭的干扰作用以及树脂的细胞毒性作用。该树脂具有抗菌活性（195.3 μg/mL ~ 1562.5 μg /mL），干扰生物膜的形成和成熟结构。树脂与oxacillin在MRSA（耐甲氧西林）分离物中的协同作用降低了抗生素的杀菌浓度。观察到分离株的粘附和侵袭显著降低（p <； 0.01）。此外，细胞活力也有小幅下降，树脂的最佳最小抑制浓度为195.3 μg / mL，仅降低了1.4 %的MAC-T活力。结果表明，青霉树脂是一种可能的抗菌草药替代品。A. cearensis树脂突出了天然产物在兽医感染中的潜力，这可以有助于减少细菌病原体的选择压力，也直接影响人类健康。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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The Microbe

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