Transcriptome analysis of Beauveria bassiana interaction with Nicotiana benthamiana reveals signatures of N. Benthamiana growth promotion and enhanced defense responses

Abstract

Many entomopathogenic fungi form intimate (epi- and endo-phytic) associations with that plant that can stimulate plant growth and /or improve resistance to pathogens and insect pests. However, little is known concerning global gene networks that mediate such responses. Nicotiana benthamiana seedlings were artificially colonized by the entomogenous fungus, Beauveria bassiana, and the root tissues were examined via comparative transcriptome analyses performed versus fungal cells grown in vitro on dried root biomass. Plant hormone pathways, and genes involved in photosynthesis, immune defense response, and nutrient metabolism were triggered in roots after fungal colonization. Fungal differentially expressed genes during plant colonization included plant cell wall-degrading enzymes, and those involved in lipid metabolism, detoxification, and fungal cell wall remodeling, the latter suggesting reduction in the exposure of pathogen related molecular patterns to avoid perception by the plant immune system. Fungal metabolic genes involved in amino acid, nitrogen, sulfur and carbohydrate assimilation were activated, nutrient exchange with the plant host. Exchange was confirmed by detection of sulfur in the seedling that was increased by the fungal colonization. A set of fungal secondary metabolism-associated genes were also upregulated during the plant interaction, which might contribute to plant resistance against pathogens or/and insect pest. In addition, B. bassiana expressed a suite of effector/elicitor genes consistent with triggering plant growth and/or immune defense response pathways. These results revealed global gene networks active in both the plants and the fungus as a consequence of their symbiotic interaction, and provides insights into the molecular determinants and physiological responses affected.