Anti-inflammatory glycoside withanolides from leaves of Athenaea velutina

IF 2.5 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia Pub Date : 2025-04-07 DOI:10.1016/j.fitote.2025.106522

Lavosyer da Silva Mendonça , Késya Amanda Dantas Rocha , Francisco das Chagas Lima Pinto , Lidiana Rabelo da Silva , Flavia Almeida Santos , Josean Fechine Tavares , Edilberto Rocha Silveira , Otília Deusdenia Loiola Pessoa

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引用次数: 0

Abstract

The phytochemical study of the CH₂Cl₂ and EtOAc soluble fractions from the leaf ethanol extract of Athenaea velutina resulted in the isolation of the new withanolides 1–3, two of which substituted at the C-3 by a disaccharide 3β-O-[α-L-rhamnopyranosyl-(1 → 4)-β-d-glucopyranosyl], and four others previously reported (4–7). The structures of the new withanolides were elucidated by detailed interpretation of their 1D- and 2D-NMR spectra and the HRESIMS analysis. An anti-inflammatory screening was performed using the two major withanolides (1 and 2), analyzing their effects on the viability of RAW 264.7 cells and their ability to inhibit cellular nitric oxide production. While withanolide 1 exhibited pro-inflammatory activity by increasing nitrite production in LPS-stimulated macrophages, compound 2, in opposition, demonstrated anti-inflammatory action by reducing those levels.

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来源期刊

Fitoterapia 医学-药学

CiteScore

5.80

自引率

2.90%

发文量

198

审稿时长

1.5 months

期刊介绍： Fitoterapia is a Journal dedicated to medicinal plants and to bioactive natural products of plant origin. It publishes original contributions in seven major areas: 1. Characterization of active ingredients of medicinal plants 2. Development of standardization method for bioactive plant extracts and natural products 3. Identification of bioactivity in plant extracts 4. Identification of targets and mechanism of activity of plant extracts 5. Production and genomic characterization of medicinal plants biomass 6. Chemistry and biochemistry of bioactive natural products of plant origin 7. Critical reviews of the historical, clinical and legal status of medicinal plants, and accounts on topical issues.