Proteasome shuttle protein DSK2b regulates the enzymatic browning of potato

IF 6.4 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology Pub Date : 2025-04-11 DOI:10.1016/j.postharvbio.2025.113566

Liqun Gong , Longhao Liu , Yuanning Zhang , Simon Stael , Huiqing Liu , Qingguo Wang , Tiantian Dong

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引用次数: 0

Abstract

Potatoes are susceptible to enzymatic browning, which affects the processing and visual quality of potato produce. The proteasome shuttle protein DSK2b was screened to be interacted with two browning-resistant protease inhibitors and DSK2b exhibited higher expression in browning-resistant potato cultivar. Further experiments found that DSK2b expression increased with browning, suggesting a potential browning-regulatory role. Therefore, the effect of DSK2b on potato browning and related mechanisms was investigated by altering DSK2b expression levels. Results showed that DSK2b-overexpression alleviated the browning of potato pulps while RNA-interference of DSK2b aggravated the phenotype. DSK2b negatively affected the free amino acid accumulation, which contributed to potato browning. Besides, DSK2b negatively regulated phenylpropanoid metabolism by modulating key enzymes of PAL, 4CL, and C4H. Concurrently, DSK2b modulated the gene expression and activities of PPO and POD. Transcriptomic and proteomic analyses revealed that DSK2b regulated potato browning, which was closely related to protease inhibitors. This study suggests that DSK2b regulates enzymatic browning in potatoes, offering a novel strategy for mitigating enzymatic browning.

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来源期刊

Postharvest Biology and Technology 农林科学-农艺学

CiteScore

12.00

自引率

11.40%

发文量

309

审稿时长

38 days

期刊介绍： The journal is devoted exclusively to the publication of original papers, review articles and frontiers articles on biological and technological postharvest research. This includes the areas of postharvest storage, treatments and underpinning mechanisms, quality evaluation, packaging, handling and distribution of fresh horticultural crops including fruit, vegetables, flowers and nuts, but excluding grains, seeds and forages. Papers reporting novel insights from fundamental and interdisciplinary research will be particularly encouraged. These disciplines include systems biology, bioinformatics, entomology, plant physiology, plant pathology, (bio)chemistry, engineering, modelling, and technologies for nondestructive testing. Manuscripts on fresh food crops that will be further processed after postharvest storage, or on food processes beyond refrigeration, packaging and minimal processing will not be considered.