Ratiometric responses of AIE/ACQ co-guests functionalized ZIF-8 nano-vehicles: Color-oriented ATP detection and intracellular imaging

IF 4.3 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-04-07 DOI:10.1016/j.saa.2025.126188

Chenyi Xue, Xuefei Zhang, Yuanyue Wu, Jiacheng Li, Tianshu Zhou, Jingjing Deng

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引用次数: 0

Abstract

In this work, two guests with opposite emission characteristics in aggregated states were rationally selected and delicately incorporated into zeolitic imidazolate framework-8 (ZIF-8) host. The simultaneous presentation aggregation-induced emission (AIE) of 1,1,2,2-Tetra (4-carboxyphenyl) ethylene (H₄TCPE) and aggregation-caused quenching (ACQ) of 5-carboxytetramethylrhodamine (5-TAMRA) endowed the as-formed H₄TCPE/5-TAMRA@ZIF-8 with dual responsive properties. Upon stimulation with ATP, the coordination environment of H₄TCPE/5-TAMRA@ZIF-8 was interrupted, leading to the collapse of ZIF-8 host and the release of guests into their free states. The corresponding quenched blue AIE at 453 nm and recovered red ACQ at 586 nm, as well as the appearance of slight monomer emission (ME) at 409 nm possibly due to weak interaction between H₄TCPE and 2-methylimidazole (Hmim), constituted a double-ratiometric sensing strategy for ATP. By plotting the fluorescence intensity ratio of (F₄₀₉ + F₅₈₆)/F₄₅₃ to the concentration of ATP ranging from 0.02-9 mM, a linear relationship was found with the limit of detection as low as 6.09 μM. This along with the good sensitivity and robust reliability of H₄TCPE/5-TAMRA@ZIF-8 probe enabled ATP detection in complex biofluids such as artificial serum and cell lysates (2.927 ± 0.565 mM) directly. Moreover, as the unique color-oriented sensing feature is inherited on the test paper, the scarcely reported H₄TCPE/5-TAMRA@ZIF-8 test strip was developed. Via the values of R/B, ATP in cell lysates can also be reported (3.1 ± 0.590 mM) easily, which may be even feasible for point-of-care diagnosis of ATP-related diseases in remote areas lacking resources. Meanwhile, as a nano-sized vehicle, H₄TCPE/5-TAMRA@ZIF-8 can easily enter cells and report intracellular ATP fluctuations by color-oriented imaging, which offers a more comprehensive and precise insight into cellular metabolism, holds significant potential for applications in bioanalytical research and environmental monitoring.

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AIE/ACQ共客体功能化ZIF-8纳米载体的比例响应：颜色取向ATP检测和细胞内成像

在这项工作中，合理选择两个具有相反发射特性的聚集态客体，并将其巧妙地结合到沸石咪唑盐框架-8 （ZIF-8）宿主中。1,1,2,2-四（4-羧基苯基）乙烯（H4TCPE）的同时呈现聚集致发射（AIE）和5-羧基四甲基罗丹明（5-TAMRA）的聚集致猝灭（ACQ）使形成的H4TCPE/5-TAMRA@ZIF-8具有双重响应特性。在ATP的刺激下，H4TCPE/5-TAMRA@ZIF-8的协调环境被中断，导致ZIF-8宿主崩溃，来宾被释放到自由状态。相应的453nm处淬灭的蓝色AIE和586 nm处恢复的红色ACQ，以及409nm处可能由于H4TCPE与2-甲基咪唑（Hmim）弱相互作用而出现的轻微单体发射（ME），构成了ATP的双比例感应策略。通过绘制(F409 + F586)/F453与ATP浓度的荧光强度比在0.02 ~ 9 mM范围内的线性关系，检测限低至6.09 μM。这与H4TCPE/5-TAMRA@ZIF-8探针良好的灵敏度和强大的可靠性一起，使ATP能够直接检测复杂的生物液体，如人工血清和细胞裂解液（2.927±0.565 mM）。此外，由于在试纸上继承了独特的面向颜色的传感特性，因此开发了很少报道的H4TCPE/5-TAMRA@ZIF-8试纸条。通过R/B值，还可以很容易地报告细胞裂解物中的ATP(3.1±0.590 mM)，甚至可以在资源缺乏的偏远地区进行ATP相关疾病的即时诊断。同时，H4TCPE/5-TAMRA@ZIF-8作为纳米尺度的载体，可以很容易地进入细胞，并通过彩色成像报告细胞内ATP的波动，从而更全面、更精确地了解细胞代谢，在生物分析研究和环境监测中具有重要的应用潜力。

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.