Structural characterization of enzymatic lignin from the saccharification of steam-exploded Eucalyptus globulus bark

IF 5.8 2区生物学 Q1 AGRICULTURAL ENGINEERING

Biomass & Bioenergy Pub Date : 2025-04-10 DOI:10.1016/j.biombioe.2025.107884

Sandra Magina, Dmitry V. Evtuguin

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Abstract

Enzymatic lignin (EL) was assessed by acidolysis from the lignocellulosic residue obtained after enzymatic saccharification of steam exploded Eucalyptus globulus bark. The structural characteristics of the obtained dioxane lignin were evaluated by wet chemistry and spectroscopy techniques (Fourier transform infrared spectroscopy, FTIR), 1D and 2D nuclear magnetic resonance (NMR) spectroscopy, and molecular weight by size exclusion chromatography (SEC). The main structural differences expressed per 100 phenyl propane units (PPU) between the dioxane lignins from the initial bark and the cellolignin residue obtained by enzymatic saccharification of the steam-exploded bark were the decrease in number of β-O-4′ structures (52 vs 40/100 PPU), increased amounts of β-β′ and β-5′ structures (11 vs 13/100 PPU) and increased molecular weight (Mw of 2400 Da vs 2700 Da) of the latter. A part of syringyl units in EL was converted into gallate-type structures as a result of partial demethoxylation of syringyl structures via homolysis that occurred during steam explosion pre-treatment.

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气爆桉树皮糖化酶促木质素的结构表征

采用酸解法对蒸汽爆破桉树皮酶解糖化后得到的木质纤维素渣进行了酶解测定。采用湿化学和光谱技术（傅里叶变换红外光谱，FTIR）、一维和二维核磁共振（NMR）、尺寸排斥色谱（SEC）和分子量分析方法对所得二氧六环木质素的结构特征进行了评价。从原始树皮中提取的二氧环木质素与通过蒸汽爆炸树皮酶解得到的纤维素木质素残渣每100苯基丙烷单位（PPU）表达的主要结构差异是β- o -4 ‘结构的数量减少（52比40/100 PPU）， β-β ’和β-5 '结构的数量增加（11比13/100 PPU），后者的分子量增加（Mw为2400 Da对2700 Da）。在蒸汽爆炸预处理过程中，丁香基结构发生均解，部分去甲氧基化，使得EL中的部分丁香基单元转化为没食子酸盐型结构。

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来源期刊

Biomass & Bioenergy 工程技术-能源与燃料

CiteScore

11.50

自引率

3.30%

发文量

258

审稿时长

60 days

期刊介绍： Biomass & Bioenergy is an international journal publishing original research papers and short communications, review articles and case studies on biological resources, chemical and biological processes, and biomass products for new renewable sources of energy and materials. The scope of the journal extends to the environmental, management and economic aspects of biomass and bioenergy. Key areas covered by the journal: • Biomass: sources, energy crop production processes, genetic improvements, composition. Please note that research on these biomass subjects must be linked directly to bioenergy generation. • Biological Residues: residues/rests from agricultural production, forestry and plantations (palm, sugar etc), processing industries, and municipal sources (MSW). Papers on the use of biomass residues through innovative processes/technological novelty and/or consideration of feedstock/system sustainability (or unsustainability) are welcomed. However waste treatment processes and pollution control or mitigation which are only tangentially related to bioenergy are not in the scope of the journal, as they are more suited to publications in the environmental arena. Papers that describe conventional waste streams (ie well described in existing literature) that do not empirically address ''new'' added value from the process are not suitable for submission to the journal. • Bioenergy Processes: fermentations, thermochemical conversions, liquid and gaseous fuels, and petrochemical substitutes • Bioenergy Utilization: direct combustion, gasification, electricity production, chemical processes, and by-product remediation • Biomass and the Environment: carbon cycle, the net energy efficiency of bioenergy systems, assessment of sustainability, and biodiversity issues.