Rapid analysis of sustainable aviation fuel precursor in a fermentation system based on in-situ Raman spectroscopy

IF 7.1 2区工程技术 Q1 ENERGY & FUELS

Sustainable Energy Technologies and Assessments Pub Date : 2025-04-01 DOI:10.1016/j.seta.2025.104305

Hui Dong , Huili Zhang , Meng Wang , Jan Baeyens , Yunming Fang

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Abstract

The ASTM have approved the hydro-processed esters and fatty acids (HEFA) and direct sugar to hydrocarbon (DSHC) bio-aviation fuel production pathway in 2011 and 2014, respectively. For the bio-aviation fuel precursor production, non-destructive and rapid in-situ detection of metabolites is of great significance for achieving the real-time monitoring and control of the fermentation process. To address the limitations of conventional analysis and detection methods, such as gas chromatography and gas chromatography-mass spectrometry, in process optimization and rapid detection, a rapid quantitative online Raman analysis of β-farnesene in fermentation and strain screening method was established, and was reported here for the first time. The testing time of β-farnesene can be reduced by at least 48 times, from 20 min to 25 s. The developed online Raman analysis method is suitable for in-situ rapid analysis and strain screening of bio-aviation fuel precursor (such as terpenes and fatty acids) with properties similar to those of β-farnesene.

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基于原位拉曼光谱的可持续航空燃料发酵体系前体快速分析

ASTM分别于2011年和2014年批准了加氢酯和脂肪酸（HEFA）和直接糖制烃（DSHC）生物航空燃料生产途径。在生物航空燃料前体生产中，代谢物的无损快速原位检测对于实现发酵过程的实时监测和控制具有重要意义。针对气相色谱、气相色谱-质谱联用等传统分析检测方法在工艺优化和快速检测方面的局限性，建立了发酵过程中β-法尼烯快速定量在线拉曼分析及菌株筛选方法，并首次报道。β-法尼烯的检测时间从20 min缩短到25 s，缩短了至少48倍。所建立的在线拉曼分析方法适用于与β-法尼烯性质相似的生物航空燃料前体（如萜烯和脂肪酸）的原位快速分析和菌株筛选。

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来源期刊

Sustainable Energy Technologies and Assessments Energy-Renewable Energy, Sustainability and the Environment

CiteScore

12.70

自引率

12.50%

发文量

1091

期刊介绍： Encouraging a transition to a sustainable energy future is imperative for our world. Technologies that enable this shift in various sectors like transportation, heating, and power systems are of utmost importance. Sustainable Energy Technologies and Assessments welcomes papers focusing on a range of aspects and levels of technological advancements in energy generation and utilization. The aim is to reduce the negative environmental impact associated with energy production and consumption, spanning from laboratory experiments to real-world applications in the commercial sector.