A VqMAPK4-VqGT3-VqNSTS6 module regulates powdery mildew resistance via stilbene biosynthesis in Chinese wild grapevine

IF 6.5 1区生物学 Q1 PLANT SCIENCES

Plant Physiology Pub Date : 2025-04-08 DOI:10.1093/plphys/kiaf140

Wandi Liu, Chaohui Yan, Zhi Li, Ruimin Li, Guotian Liu, Yuejin Wang

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Abstract

Grapes are widely cultivated around the world and valued for their rich nutritional content and versatile use in various industries. However, grape powdery mildew (PM) threatens grape production. This study aimed to identify and characterize PM resistance genes in Chinese wild grapevine (Vitis quinquangularis). Grape powdery mildew resistance is associated with stilbene synthases (STSs). Here, we isolated VqNSTS6 from the Chinese wild grapevine accession ‘Danfeng-2’ that exhibits high PM resistance. Overexpression of VqNSTS6 in the susceptible ‘Thompson Seedless’ variety induced PM resistance, whereas its transient knockdown in ‘Danfeng-2’ diminished this resistance. Furthermore, VqNSTS6 expression was upregulated by VqGT3, resulting in stilbene accumulation and enhanced PM resistance. However, stilbene overaccumulation induced Mitogen-Activated Protein Kinase 4 (MAPK4) phosphorylation, which in turn triggered VqGT3 phosphorylation and degradation, consequently downregulating VqNSTS6 and mitigating excessive stilbene accumulation. Additionally, VqNSTS6-GFP moved toward and wrapped around pathogen haustoria, forming a barrier preventing Golovinomyces cichoracearum invasion of Arabidopsis (Arabidopsis thaliana). The characterization of the STS gene VqNSTS6 conferring PM resistance opens avenues for breeding PM-resistant grapevine genotypes.

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VqMAPK4-VqGT3-VqNSTS6 模块通过二苯乙烯生物合成调控中国野生葡萄的白粉病抗性

葡萄在世界各地广泛种植，因其丰富的营养成分和在各种工业中的广泛用途而受到重视。然而，葡萄白粉病威胁着葡萄的生产。本研究旨在鉴定和鉴定中国野生葡萄（Vitis quinquangularis）抗PM基因。葡萄白粉病抗性与二苯乙烯合成酶（STSs）有关。在此，我们从具有高PM抗性的中国野生葡萄品种‘丹丰2号’中分离到了VqNSTS6。VqNSTS6在易感的“汤普森无籽”品种中过表达诱导了PM抗性，而在“丹风2号”中瞬时敲低则减弱了这种抗性。此外，VqGT3可上调VqNSTS6的表达，导致芪积累，增强抗PM能力。然而，stilbene过度积累诱导mitoogen - activated Protein Kinase 4 （MAPK4）磷酸化，进而引发VqGT3磷酸化和降解，从而下调VqNSTS6，缓解stilbene过度积累。此外，VqNSTS6-GFP向病原体吸体移动并包裹在吸体周围，形成了一个屏障，阻止了拟南芥（Arabidopsis thaliana）对拟南芥的入侵。具有抗PM抗性的STS基因VqNSTS6的鉴定为培育抗PM葡萄基因型开辟了途径。

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来源期刊

Plant Physiology 生物-植物科学

CiteScore

12.20

自引率

5.40%

发文量

535

审稿时长

2.3 months

期刊介绍： Plant Physiology® is a distinguished and highly respected journal with a rich history dating back to its establishment in 1926. It stands as a leading international publication in the field of plant biology, covering a comprehensive range of topics from the molecular and structural aspects of plant life to systems biology and ecophysiology. Recognized as the most highly cited journal in plant sciences, Plant Physiology® is a testament to its commitment to excellence and the dissemination of groundbreaking research. As the official publication of the American Society of Plant Biologists, Plant Physiology® upholds rigorous peer-review standards, ensuring that the scientific community receives the highest quality research. The journal releases 12 issues annually, providing a steady stream of new findings and insights to its readership.