Neospora caninum in goats from Iraq: A serological and molecular study.

IF 0.9 Q3 VETERINARY SCIENCES

Open Veterinary Journal Pub Date : 2025-02-01 Epub Date: 2025-02-28 DOI:10.5455/OVJ.2025.v15.i2.21

Roqaya B Mohammed, Mansour J Ali

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Abstract

Background: Neospora caninum is an Apicomplexa parasite that is an important abortive infectious agent in cattle, goats, and other domestic and wild animals.

Aim: This study aimed to identify N. caninum using Enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) in suspected goats in Al-Diwaniyah Province, Iraq.

Methods: This study was conducted to identify N. caninum in blood and milk samples of 138 goats (92 blood and 46 milk samples) from suspected infected goats in Al-Diwaniyah Province, Iraq. Two techniques were used; ELISA and PCR (NC5-gene-based and partial sequencing), and the recorded data were categorized into age, sex, and region. Results: ELISA results revealed that the seroprevalence rates were 14.13% (13/92) in blood and 21.73% (10/46) in milk samples. The PCR results demonstrated that for the blood samples, 3/45 (6.66%) and 2/47 (4.25%) were positive for males and females, respectively. The PCR milk findings showed that the highest rates were 3/20 (15%) and 1/26 (3.84%) in the 2-4 and 4-6 age groups, respectively. Sequencing demonstrated close identities of the examined isolates to those from New Zealand and Australia for the blood and milk samples.

Conclusions: The findings of this study reveal that N. caninum is widely distributed in goats, which could be an important source of zoonosis in the tested areas. The phylogenetic analysis shows a shared ancestor between the study isolates and those from New Zealand and Australia.

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伊拉克山羊的犬新孢子虫：血清学和分子研究。

背景：犬新孢子虫是一种顶复合体寄生虫，是牛、山羊及其他家畜和野生动物的重要致病菌。目的：利用酶联免疫吸附试验（ELISA）和聚合酶链反应（PCR）对伊拉克Al-Diwaniyah省可疑山羊进行犬奈索菌鉴定。方法：对伊拉克Al-Diwaniyah省138只山羊（92只山羊血，46只山羊奶）血液和羊奶标本进行犬奈尔菌鉴定。使用了两种技术；ELISA和PCR（基于nc5基因和部分测序），并将记录的数据按年龄、性别和地区分类。结果：ELISA检测结果显示血血清阳性率为14.13%(13/92)，牛奶血清阳性率为21.73%（10/46）。PCR结果显示，男性阳性3/45(6.66%)，女性阳性2/47（4.25%）。PCR牛奶检测结果显示，2 ~ 4岁和4 ~ 6岁年龄组的感染率分别为3/20（15%）和1/26（3.84%）。测序结果表明，所检测的分离株与来自新西兰和澳大利亚的血液和牛奶样本的分离株具有密切的同一性。结论：本研究结果表明，犬奈尔菌在山羊中广泛分布，可能是疫区人畜共患病的重要传染源。系统发育分析表明，研究分离株与来自新西兰和澳大利亚的分离株有共同的祖先。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Open Veterinary Journal VETERINARY SCIENCES-

CiteScore

1.40

自引率

0.00%

发文量

112

审稿时长

12 weeks

期刊介绍： Open Veterinary Journal is a peer-reviewed international open access online and printed journal that publishes high-quality original research articles. reviews, short communications and case reports dedicated to all aspects of veterinary sciences and its related subjects. Research areas include the following: Infectious diseases of zoonotic/food-borne importance, applied biochemistry, parasitology, endocrinology, microbiology, immunology, pathology, pharmacology, physiology, epidemiology, molecular biology, immunogenetics, surgery, ophthalmology, dermatology, oncology and animal reproduction. All papers are peer-reviewed. Moreover, with the presence of well-qualified group of international referees, the process of publication will be done meticulously and to the highest standards.