Sedimentitalea sediminis sp. nov., a novel bacterium isolated from marine sediment.

Abstract

Strain HM32M-2 ^T, an aerobic, catalase-positive, oxidase-positive, and Gram-stain-negative bacterium, was isolated from a sandy sediment sample collected from Qinzhou Gulf in Guangxi Zhuang Autonomous Region, China. Strain HM32M-2 ^T grew at 15-37 °C (optimum, 30 °C), at pH 5.5-9.5 (optimum, pH 8.5), and with 1.0-12.0% (w/v) NaCl (optimum, 3.0%). Strain HM32M-2 ^T exhibited the highest 16S rRNA gene sequence similarity with Sedimentitalea nanhaiensis NH52F^T (98.4%). Phylogenetic analyses based on 16S rRNA gene sequences and whole-genome sequences showed that strain HM32M-2 ^T formed a distinct lineage with Sedimentitalea nanhaiensis NH52F^T. The draft genome of strain HM32M-2 ^T was 3.40 Mbp in size and its DNA G + C content was 63.6%. Comparative genome analysis revealed that average nucleotide identity and digital DNA-DNA hybridization among strain HM32M-2 ^T and other Sedimentitalea species were below cut-off levels of 95-96% and 70%, respectively. Chemotaxonomic analyses indicated that strain HM32M-2 ^T contained Q-10 as the respiratory quinone, C_18:1ω7c as the major cellular fatty acid, and diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, one unidentified aminolipid, and one unidentified lipid as the major polar lipids. Strain HM32M-2 ^T had a typical chemical composition of fatty acids, polar lipids, and quinones for Sedimentitalea species, but could be distinguished from known species of the genus Sedimentitalea. On the basis of the polyphasic evidence, strain HM32M-2 ^T should be classified as a novel species of the genus Sedimentitalea, for which the name Sedimentitalea sediminis sp. nov. is proposed. The type strain is HM32M-2 ^T (= MCCC 1K08873^T = KCTC 8272 ^T).