Genome-wide identification and expression analysis of the TCP transcription factor family and its response to abiotic stress in rapeseed (Brassica napus L.).

Abstract

The study used 80 BnTCP genes (Brassica napus TCP genes) in rapeseed, which were identified and designated with nomenclature based on their chromosomal locations. A systematic analysis encompassed the evolutionary relationships, classifications, gene structures, motif compositions, chromosome localization, and gene replication events within these BnTCP genes. These 80 BnTCP proteins were categorized into three subfamilies, with the PCF subfamily showing significant expansion during evolution. Segmental duplications were identified as a major driver of TCP family amplification. To comprehensively assess the evolutionary relationships of the TCP family across diverse plant species, nine comparative genomic maps were constructed, elucidating homologous genes between B. napus and representative monocotyledonous and dicotyledonous plants. In the final phase of the study, the gene expression response characteristics of 15 selected BnTCP genes across various biological processes and stress responses were examined. Noteworthy candidates, including BnTCP28, BnTCP30, and BnTCP76, were identified as potentially crucial in tissue development and environmental stress responses.

Supplementary information: The online version contains supplementary material available at 10.1007/s13205-025-04273-x.