Superior peroxidase-like activities of InVO4 hollow nanocuboid assemblies for colorimetric detection of hydrogen peroxide and glucose

Abstract

In this work, InVO₄ hollow nanocuboid assemblies (IVHNs) with larger specific surface area were synthesized by a facile one-pot hydrothermal method, which were utilized as artificial biomimetic catalysts. In the presence of hydrogen peroxide (H₂O₂), the IVHNs nanozyme enchantingly displayed an extraordinary peroxidase-like performance, inducing the oxidation of the chromogenic compound 3,3′,5,5′-tetramethylbenzidine (TMB) to generate a blue oxide. Kinetics analysis unequivocally demonstrated that the catalytic behavior of IVHNs conformed to the Michaelis-Menten mechanism, and ·O₂⁻ radicals played a vital role in the catalytic process according to the active species capture experiments. Given the exceptional catalytic performance of IVHNs, a colorimetric sensing platform was successfully established for rapid and accurate detection of H₂O₂ and glucose. The concentrations of H₂O₂ and glucose were positively correlated with the absorbance of TMB oxide in the linear ranges of 1–25 μM and 10–100 μM with the detection limit of 0.092 μM and 0.96 μM, respectively. Enriched by its exquisite stability, selectivity, and anti-interference ability, the colorimetric sensing system bestowed its success upon qualitative detection of H₂O₂ and glucose in real samples. This study not only unveils a novel nanozyme boasting remarkably efficient catalytic prowess, but also pioneers a rapid and straightforward approach for environmental analysis and clinical diagnosis.