Ameliorating vascular endothelial injury for lipolysacharide-induced via mitochondrial targeting function of octaarginine-modified essential oil from Fructus Alpiniae zerumbet (EOFAZ) lipid microspheres

IF 4.7 4区医学 Q1 CHEMISTRY, MEDICINAL

Chinese Herbal Medicines Pub Date : 2025-04-01 DOI:10.1016/j.chmed.2024.11.004

Lingyan Li , Zengqiu Yang , Qiqi Li , Qianqian Guo , Xingjie Wu , Yu’e Wang , Xiangchun Shen , Ying Chen , Ling Tao

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引用次数: 0

Abstract

Objective

To investigate the therapeutic potential of octaarginine (R8)-modified essential oil from Fructus Alpiniae zerumbet (EOFAZ) lipid microspheres (EOFAZ@^R8LM) for cardiovascular therapy.

Methods

EOFAZ@^R8LM was developed by leveraging the volatilization of EOFAZ and integrating it with the oil phase of LM, followed by surface modification with cell-penetrating peptide R8 to target the site of vascular endothelial injury. The therapeutic effects of this formulation in alleviating lipopolysaccharide-induced vascular endothelial inflammation were evaluated by assessing mitochondrial membrane potential (MMP), intracellular reactive oxygen species (ROS) levels, as well as inflammatory factors interleukin-6 (IL-6) and interleukin-1β (IL-1β) levels.

Results

EOFAZ@^R8LM effectively delivered EOFAZ to the site of injury and specifically targeted the mitochondria in vascular endothelial cells, thereby ameliorating mitochondrial dysfunction through regulation of MMP and reduction of intracellular ROS levels. Moreover, it attenuated the expression levels of IL-6 and IL-1β, exerting protective effects on the vascular endothelium.

Conclusion

Our findings highlight the significant therapeutic potential of EOFAZ@^R8LM in cardiovascular therapy, providing valuable insights for developing novel dosage forms utilizing EOFAZ for effective treatment against cardiovascular diseases.

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八精氨酸修饰的荆果精油脂质微球对脂多糖诱导的血管内皮损伤的线粒体靶向作用

目的探讨八精氨酸（R8）修饰的荆果精油（EOFAZ）脂质微球（EOFAZ@R8LM）治疗心血管疾病的潜力。MethodsEOFAZ@R8LM是利用EOFAZ的挥发性，将其与LM的油相结合，然后用细胞穿透肽R8对其表面进行修饰，靶向血管内皮损伤部位而开发的。通过评估线粒体膜电位（MMP）、细胞内活性氧（ROS）水平以及炎症因子白介素-6 （IL-6）和白介素-1β (IL-1β)水平，评估该制剂缓解脂多糖诱导的血管内皮炎症的治疗效果。ResultsEOFAZ@R8LM有效地将EOFAZ递送至损伤部位，并特异性靶向血管内皮细胞中的线粒体，从而通过调节MMP和降低细胞内ROS水平改善线粒体功能障碍。降低IL-6和IL-1β的表达水平，对血管内皮具有保护作用。结论本研究结果突出了EOFAZ@R8LM在心血管疾病治疗中的显著治疗潜力，为开发利用EOFAZ有效治疗心血管疾病的新剂型提供了有价值的见解。

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来源期刊

Chinese Herbal Medicines CHEMISTRY, MEDICINAL-

CiteScore

4.40

自引率

5.30%

发文量

629

审稿时长

10 weeks

期刊介绍： Chinese Herbal Medicines is intended to disseminate the latest developments and research progress in traditional and herbal medical sciences to researchers, practitioners, academics and administrators worldwide in the field of traditional and herbal medicines. The journal's international coverage ensures that research and progress from all regions of the world are widely included. CHM is a core journal of Chinese science and technology. The journal entered into the ESCI database in 2017, and then was included in PMC, Scopus and other important international search systems. In 2019, CHM was successfully selected for the “China Science and Technology Journal Excellence Action Plan” project, which has markedly improved its international influence and industry popularity. CHM obtained the first impact factor of 3.8 in Journal Citation Reports (JCR) in 2023.