Two new polyketides from Rhodiola tibetica endophytic fungus Penicillium sp. HJT-A-6

IF 4.7 4区医学 Q1 CHEMISTRY, MEDICINAL

Chinese Herbal Medicines Pub Date : 2025-04-01 DOI:10.1016/j.chmed.2024.06.004

Dongliang Xiao , Xiaobao Li , Xuemei Zhang , Nan Jiang , Dunzhu Luosang , Weixing Feng , Xuan Lu , Baomin Feng

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引用次数: 0

Abstract

Objective

To study bioactive compounds from the endophytic fungus Penicillium sp. HJT-A-6 isolated from stem of Rhodiola tibetica, and evaluate its allelopathic activity.

Methods

The chemical constituents were isolated and purified by silica gel, Sephadex LH-20 column chromatography and semi-preparative HPLC. Their structures were elucidated by extensive spectroscopic analysis and electronic circular dichroism (ECD) calculations. In addition, the allelopathic activity of compound 1 was evaluated by measuring the seed germination rate of R. tibetica.

Results

Two new polyketides 4-hydroxy-3,6-dimethyl-2H-pyran-2-one (1) and penilactone E (2), together with six known compounds walterolactone A (3), 5-hydroxyhexan-4-olide (4), 3-methyl-2-penten-5-olide (5), chaetoquadrin F (6), (Z)-6-acetyl-3-(1,2-dihydroxypropylidene)-5-hydroxy-8-methylchroman-2-one (7) and 4-hydroxy-3-(4-hydroxyhexanoyl)-5-methylfuran-2(5H)-one (8) were isolated from Penicillium sp. HJT-A-6. Compound 1 showed moderate seed-germination-promoting activity at a concentration of 0.001 mg/mL while inhibiting the seed germination at concentrations of 0.1 and 0.01 mg/mL. Compared with the positive drug 6-benzyladenine (6-BA), compound 1 could extend the seed-germination period of R. tibetica (up to 11 d).

Conclusion

Two new compounds were isolated from R. tibetica endophytic fungus Penicillium sp. HJT-A-6. Compound 1 displayed plant hormone-like activity, which inhibited the seed germination of the host plant at high concentrations and promoted the seed germination of the host plant at low concentrations. The results not only enrich the chemical constituents of the endophytic fungi isolated from Rhodiola tibetica, but also provide a theoretical basis for understanding the interaction mechanism between Rhodiola tibetica endophytic fungi and the host plant.

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西藏红景天内生真菌青霉菌HJT-A-6的两个新聚酮

方法通过硅胶、Sephadex LH-20 柱色谱法和半制备高效液相色谱法分离和纯化化学成分。通过大量光谱分析和电子圆二色性（ECD）计算，阐明了它们的结构。此外，化合物 1 的等位活性还通过测定 R. tibetica 的种子萌发率进行了评估。结果两种新的多酮类化合物 4-羟基-3,6-二甲基-2H-吡喃-2-酮（1）和五内酯 E（2），以及六种已知化合物胡桃内酯 A（3）、5-羟基己烷-4-内酯（4）、3-甲基-2-戊烯-5-内酯（5）、从青霉 sp.HJT-A-6。化合物 1 在浓度为 0.001 毫克/毫升时显示出中等程度的促进种子萌发活性，而在浓度为 0.1 和 0.01 毫克/毫升时则抑制种子萌发。与阳性药物 6-苄基腺嘌呤（6-BA）相比，化合物 1 可延长铁线莲种子的发芽期（长达 11 d）。化合物 1 具有类似植物激素的活性，高浓度时抑制寄主植物种子的萌发，低浓度时促进寄主植物种子的萌发。研究结果不仅丰富了从红景天中分离的内生真菌的化学成分，而且为理解红景天内生真菌与寄主植物之间的相互作用机制提供了理论依据。

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来源期刊

Chinese Herbal Medicines CHEMISTRY, MEDICINAL-

CiteScore

4.40

自引率

5.30%

发文量

629

审稿时长

10 weeks

期刊介绍： Chinese Herbal Medicines is intended to disseminate the latest developments and research progress in traditional and herbal medical sciences to researchers, practitioners, academics and administrators worldwide in the field of traditional and herbal medicines. The journal's international coverage ensures that research and progress from all regions of the world are widely included. CHM is a core journal of Chinese science and technology. The journal entered into the ESCI database in 2017, and then was included in PMC, Scopus and other important international search systems. In 2019, CHM was successfully selected for the “China Science and Technology Journal Excellence Action Plan” project, which has markedly improved its international influence and industry popularity. CHM obtained the first impact factor of 3.8 in Journal Citation Reports (JCR) in 2023.