Development of neutralization tests using single-round infectious particles and cytopathic effect as an alternative method for measuring antibody titers against Japanese encephalitis virus in national epidemiological surveillance program of vaccine-preventable diseases in Japan

IF 2.2 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS

Journal of virological methods Pub Date : 2025-04-08 DOI:10.1016/j.jviromet.2025.115163

Shunsuke Yazawa , Yumiko Saga , Mami Matsuda , Ryosuke Suzuki , Shigeru Tajima , Chang-Kweng Lim , Hideki Tani

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引用次数: 0

Abstract

The “National Epidemiological Surveillance of Vaccine-Preventable Diseases” is conducted annually in Japan for various infectious diseases. A susceptibility survey for Japanese encephalitis was conducted by measuring antibody titers using a focus-forming assay. However, this method is complicated to operate and hard to count small focuses; therefore, the development of new measurement methods is required. In this study, we developed and evaluated a single-round infectious particles (SRIPs) assay method and a cytopathic effect (CPE) assay method. The SRIPs assay showed a strong correlation (R = 0.94) with the focus-forming assay. The SRIPs assay method is not only simple and can be expected to improve accuracy, but also has the advantages of extremely low pathogenicity and safety for the operator. The CPE assay also showed a strong correlation (R = 0.80) with the focus-forming assay. It has the advantage of requiring almost no reagents and is easy to interpret. Both SRIPs and CPE assay methods can be used as alternatives to the focus-forming assay method, but further characterization of each method is necessary before one is selected for routine use.

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来源期刊

Journal of virological methods 医学-病毒学

CiteScore

5.80

自引率

0.00%

发文量

209

审稿时长

41 days

期刊介绍： The Journal of Virological Methods focuses on original, high quality research papers that describe novel and comprehensively tested methods which enhance human, animal, plant, bacterial or environmental virology and prions research and discovery. The methods may include, but not limited to, the study of: Viral components and morphology- Virus isolation, propagation and development of viral vectors- Viral pathogenesis, oncogenesis, vaccines and antivirals- Virus replication, host-pathogen interactions and responses- Virus transmission, prevention, control and treatment- Viral metagenomics and virome- Virus ecology, adaption and evolution- Applied virology such as nanotechnology- Viral diagnosis with novelty and comprehensive evaluation. We seek articles, systematic reviews, meta-analyses and laboratory protocols that include comprehensive technical details with statistical confirmations that provide validations against current best practice, international standards or quality assurance programs and which advance knowledge in virology leading to improved medical, veterinary or agricultural practices and management.