Yanan Peng, Qiumei Pu, Liangqing Lu, Qionglin Zhou, Xinxin Xiao, Xiangde Lai, Xuan Zhao, Bin Qiao, Qiang Wu
{"title":"Erasable Fluorescence Imaging Technology Enables Continuous Tracking for Identical Living Cells","authors":"Yanan Peng, Qiumei Pu, Liangqing Lu, Qionglin Zhou, Xinxin Xiao, Xiangde Lai, Xuan Zhao, Bin Qiao, Qiang Wu","doi":"10.1002/anie.202503818","DOIUrl":null,"url":null,"abstract":"<p>Subtle molecular events may trigger a cascade of “butterfly effects” to reverse cell fate, so dynamic tracking of relevant markers can provide crucial clues to decipher the unknown. However, existing analytical techniques are still at static-analysis level, and cutting-edge fluorescence imaging is limited by signal interference at adjacent time, failing to realize continuous observation for identical live cells. Herein, we develop a new temperature-controlled imaging technology, where melamine-mediated reversible DNA self-assembly drives the fluorescence illuminating and extinguishing at any time, achieving repeated erasable imaging for identical living cells. This method can track cell behaviors by taking dynamic monitoring of cell differentiation as an example, and timely fluorescence erasing guarantees authenticity for imaging results and minimizes interference with cellular activities. Thanks to high feasibility in fundamental experimental scenarios, this method hopefully provides a powerful preliminary screening tool for exploring new biological mechanisms in cell interactions, developmental transformations, downstream response inquiry, and so forth.</p>","PeriodicalId":125,"journal":{"name":"Angewandte Chemie International Edition","volume":"64 25","pages":""},"PeriodicalIF":16.1000,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Angewandte Chemie International Edition","FirstCategoryId":"92","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/anie.202503818","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
Subtle molecular events may trigger a cascade of “butterfly effects” to reverse cell fate, so dynamic tracking of relevant markers can provide crucial clues to decipher the unknown. However, existing analytical techniques are still at static-analysis level, and cutting-edge fluorescence imaging is limited by signal interference at adjacent time, failing to realize continuous observation for identical live cells. Herein, we develop a new temperature-controlled imaging technology, where melamine-mediated reversible DNA self-assembly drives the fluorescence illuminating and extinguishing at any time, achieving repeated erasable imaging for identical living cells. This method can track cell behaviors by taking dynamic monitoring of cell differentiation as an example, and timely fluorescence erasing guarantees authenticity for imaging results and minimizes interference with cellular activities. Thanks to high feasibility in fundamental experimental scenarios, this method hopefully provides a powerful preliminary screening tool for exploring new biological mechanisms in cell interactions, developmental transformations, downstream response inquiry, and so forth.
期刊介绍:
Angewandte Chemie, a journal of the German Chemical Society (GDCh), maintains a leading position among scholarly journals in general chemistry with an impressive Impact Factor of 16.6 (2022 Journal Citation Reports, Clarivate, 2023). Published weekly in a reader-friendly format, it features new articles almost every day. Established in 1887, Angewandte Chemie is a prominent chemistry journal, offering a dynamic blend of Review-type articles, Highlights, Communications, and Research Articles on a weekly basis, making it unique in the field.