Erasable Fluorescence Imaging Technology Enables Continuous Tracking for Identical Living Cells

IF 16.1 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Yanan Peng, Qiumei Pu, Liangqing Lu, Qionglin Zhou, Xinxin Xiao, Xiangde Lai, Xuan Zhao, Bin Qiao, Qiang Wu
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引用次数: 0

Abstract

Subtle molecular events may trigger a cascade of “butterfly effects” to reverse cell fate, so dynamic tracking of relevant markers can provide crucial clues to decipher the unknown. However, existing analytical techniques are still at static-analysis level, and cutting-edge fluorescence imaging is limited by signal interference at adjacent time, failing to realize continuous observation for identical live cells. Herein, we develop a new temperature-controlled imaging technology, where melamine-mediated reversible DNA self-assembly drives the fluorescence illuminating and extinguishing at any time, achieving repeated erasable imaging for identical living cells. This method can track cell behaviors by taking dynamic monitoring of cell differentiation as an example, and timely fluorescence erasing guarantees authenticity for imaging results and minimizes interference with cellular activities. Thanks to high feasibility in fundamental experimental scenarios, this method hopefully provides a powerful preliminary screening tool for exploring new biological mechanisms in cell interactions, developmental transformations, downstream response inquiry, and so forth.

Abstract Image

可擦除荧光成像技术可以连续跟踪相同的活细胞
微妙的分子事件可能引发一连串的“蝴蝶效应”,从而逆转细胞命运,因此对相关标记的动态跟踪可以为破译未知提供关键线索。然而,现有的分析技术还停留在静态分析水平,前沿的荧光成像受相邻时间信号干扰的限制,无法实现对相同活细胞的连续观察。在此,我们开发了一种新的温度控制成像技术,其中三聚氰胺介导的可逆DNA自组装驱动荧光随时点亮和熄灭,实现了对相同活细胞的重复可擦除成像。该方法以动态监测细胞分化为例,可以对细胞行为进行跟踪,及时的荧光擦除保证了成像结果的真实性,最大限度地减少了对细胞活动的干扰。该方法在基础实验场景中具有较高的可行性,有望为探索细胞相互作用、发育转化、下游反应探究等新的生物学机制提供强大的初步筛选工具。
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来源期刊
CiteScore
26.60
自引率
6.60%
发文量
3549
审稿时长
1.5 months
期刊介绍: Angewandte Chemie, a journal of the German Chemical Society (GDCh), maintains a leading position among scholarly journals in general chemistry with an impressive Impact Factor of 16.6 (2022 Journal Citation Reports, Clarivate, 2023). Published weekly in a reader-friendly format, it features new articles almost every day. Established in 1887, Angewandte Chemie is a prominent chemistry journal, offering a dynamic blend of Review-type articles, Highlights, Communications, and Research Articles on a weekly basis, making it unique in the field.
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