Picrosirius Red Staining for Semiquantitative Histopathologic Evaluation of Collagen Deposition in Murine Models of Chronic Lung Allograft Rejection.

IF 1.2 4区综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES

Jove-Journal of Visualized Experiments Pub Date : 2025-03-21 DOI:10.3791/66395

Birte Ohm, Julia Thomé, Thierry Christmann, Maike Lind, Yoshito Yamada, Andreas Spörlein, Laura Anna Schneider, Steffen U Eisenhardt, Wolfgang Jungraithmayr

{"title":"Picrosirius Red Staining for Semiquantitative Histopathologic Evaluation of Collagen Deposition in Murine Models of Chronic Lung Allograft Rejection.","authors":"Birte Ohm, Julia Thomé, Thierry Christmann, Maike Lind, Yoshito Yamada, Andreas Spörlein, Laura Anna Schneider, Steffen U Eisenhardt, Wolfgang Jungraithmayr","doi":"10.3791/66395","DOIUrl":null,"url":null,"abstract":"<p><p>Fibrosis is the pathophysiologic hallmark of chronic rejection after lung transplantation and the foremost hurdle to long-term recipient survival. Several murine lung transplantation models are available for the study of chronic rejection. However, they display heterogeneous results regarding fibrotic changes of the graft, and the histologic extent of fibrosis is mostly reported qualitatively. Therefore, a spatially resolved approach that allows for statistical analysis can aid in evaluating fibrosis in these models. This study presents Picrosirius Red staining for a semiquantitative evaluation of collagen organization in murine lung allografts and compares it to standard Hematoxylin and Eosin, Masson's Trichrome, and Herovici's stains. Staining was performed on sections from two different murine transplantation models based on minor and major histocompatibility complex (MHC) mismatches. The method was established for semiquantitative analysis of collagen organization in whole lung sections. Thus, it can serve as a tool for murine experimental models of fibrotic lung diseases.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 217","pages":""},"PeriodicalIF":1.2000,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Jove-Journal of Visualized Experiments","FirstCategoryId":"103","ListUrlMain":"https://doi.org/10.3791/66395","RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Fibrosis is the pathophysiologic hallmark of chronic rejection after lung transplantation and the foremost hurdle to long-term recipient survival. Several murine lung transplantation models are available for the study of chronic rejection. However, they display heterogeneous results regarding fibrotic changes of the graft, and the histologic extent of fibrosis is mostly reported qualitatively. Therefore, a spatially resolved approach that allows for statistical analysis can aid in evaluating fibrosis in these models. This study presents Picrosirius Red staining for a semiquantitative evaluation of collagen organization in murine lung allografts and compares it to standard Hematoxylin and Eosin, Masson's Trichrome, and Herovici's stains. Staining was performed on sections from two different murine transplantation models based on minor and major histocompatibility complex (MHC) mismatches. The method was established for semiquantitative analysis of collagen organization in whole lung sections. Thus, it can serve as a tool for murine experimental models of fibrotic lung diseases.

查看原文本刊更多论文

小天狼星红染色对小鼠慢性同种异体肺移植排斥反应模型中胶原沉积的半定量组织病理学评价。

纤维化是肺移植后慢性排斥反应的病理生理标志，也是影响受体长期生存的首要障碍。几种小鼠肺移植模型可用于慢性排斥反应的研究。然而，它们在移植物的纤维化变化方面显示出不同的结果，并且纤维化的组织学程度主要是定性报道。因此，允许进行统计分析的空间分解方法可以帮助评估这些模型中的纤维化。本研究采用小天狼星红染色法半定量评价小鼠肺同种异体移植物的胶原组织，并将其与标准苏木精和伊红、马松三色和Herovici染色法进行比较。根据组织相容性复合体（MHC）轻微和主要不匹配，对两种不同小鼠移植模型的切片进行染色。建立了半定量分析全肺切片胶原组织的方法。因此，它可以作为小鼠纤维化肺疾病实验模型的工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Jove-Journal of Visualized Experiments MULTIDISCIPLINARY SCIENCES-

CiteScore

2.10

自引率

0.00%

发文量

992

期刊介绍： JoVE, the Journal of Visualized Experiments, is the world''s first peer reviewed scientific video journal. Established in 2006, JoVE is devoted to publishing scientific research in a visual format to help researchers overcome two of the biggest challenges facing the scientific research community today; poor reproducibility and the time and labor intensive nature of learning new experimental techniques.