Spindle observation aids decision-making for early rescue ICSI.

IF 2.7 3区医学 Q2 GENETICS & HEREDITY

Journal of Assisted Reproduction and Genetics Pub Date : 2025-05-01 Epub Date: 2025-04-07 DOI:10.1007/s10815-025-03474-w

Yang Gao, Jiahong Zhu, Shun Xiong, Jiang Wang, Xiangwei Hao, Haiyuan Liao, Wei Han, Junxia Liu, Guoning Huang

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引用次数: 0

Abstract

Objective: To investigate whether spindle visualization using polarized light microscopy (PLM) can assist in identifying unfertilized oocytes prior to early rescue intracytoplasmic sperm injection (R-ICSI).

Methods: This retrospective cohort study included 356 early R-ICSI cycles, of which 24 were excluded from the analysis. The remaining 332 cycles were divided into study group and control group based on whether spindle observation had been performed before early R-ICSI. The fertilization rate, embryo development, and clinical outcome were compared between the two groups.

Results: Of the 356 participants included, 332 completed follow-up analysis. The study group demonstrated a significantly higher normal fertilization rate (82.55% vs. 71.49%, P < 0.01) and a lower 3PN rate (3.17% vs. 8.89%, P < 0.01) after following R-ICSI. For oocytes without visible spindles in the study group or those with fragmented polar bodies in the control group, in vitro fertilization (IVF) was performed, resulting in a higher normal fertilization rate in the study group (53.37% vs. 36.43%, P < 0.01). However, there was no significant difference between the two groups in terms of embryo development and implantation potential. In the high-age subgroup (over 35 years old), the normal cleavage rate was significantly higher in the spindle examination group compared to the non-observation group (38.60% vs. 22.47%, P < 0.05).

Conclusion: Utilizing PLM for spindle observation can effectively identify oocytes suitable for early R-ICSI to improve fertilization outcomes and optimize embryo development in women over 35 years old.

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纺锤体观察有助于早期ICSI抢救决策。

目的：探讨利用偏振光显微镜（PLM）观察纺锤体是否有助于早期抢救性胞浆内单精子注射（R-ICSI）前识别未受精卵母细胞。方法：本回顾性队列研究包括356例早期R-ICSI周期，其中24例被排除在分析之外。其余332个周期根据早期R-ICSI前是否进行纺锤体观察分为研究组和对照组。比较两组受精率、胚胎发育及临床结局。结果：在356名参与者中，332人完成了随访分析。研究组经R-ICSI后正常受精率（82.55% vs. 71.49%, P < 0.01）显著高于对照组（P < 0.01）， 3PN率（3.17% vs. 8.89%, P < 0.01）显著低于对照组（P < 0.01）。对研究组未见纺锤体或对照组极体破碎的卵母细胞进行体外受精（IVF），研究组正常受精率高于对照组（53.37% vs. 36.43%, P < 0.01）。然而，两组在胚胎发育和着床潜能方面无显著差异。在高年龄亚组（35岁以上）中，纺锤体检查组正常卵裂率显著高于未观察组（38.60%比22.47%,P < 0.05）。结论：利用PLM观察纺锤体可有效识别适合早期R-ICSI的卵母细胞，改善35岁以上妇女的受精效果，优化胚胎发育。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Assisted Reproduction and Genetics 医学-妇产科学

CiteScore

5.70

自引率

9.70%

发文量

286

审稿时长

1 months

期刊介绍： The Journal of Assisted Reproduction and Genetics publishes cellular, molecular, genetic, and epigenetic discoveries advancing our understanding of the biology and underlying mechanisms from gametogenesis to offspring health. Special emphasis is placed on the practice and evolution of assisted reproduction technologies (ARTs) with reference to the diagnosis and management of diseases affecting fertility. Our goal is to educate our readership in the translation of basic and clinical discoveries made from human or relevant animal models to the safe and efficacious practice of human ARTs. The scientific rigor and ethical standards embraced by the JARG editorial team ensures a broad international base of expertise guiding the marriage of contemporary clinical research paradigms with basic science discovery. JARG publishes original papers, minireviews, case reports, and opinion pieces often combined into special topic issues that will educate clinicians and scientists with interests in the mechanisms of human development that bear on the treatment of infertility and emerging innovations in human ARTs. The guiding principles of male and female reproductive health impacting pre- and post-conceptional viability and developmental potential are emphasized within the purview of human reproductive health in current and future generations of our species. The journal is published in cooperation with the American Society for Reproductive Medicine, an organization of more than 8,000 physicians, researchers, nurses, technicians and other professionals dedicated to advancing knowledge and expertise in reproductive biology.