Examining the potential of plastic-fed black soldier fly larvae (Hermetia illucens) as "bioincubators" of plastic-degrading bacteria.

IF 3.2 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Nicholas B Dragone, Sophie van Hamelsveld, Ali Reza Nazmi, Matthew Stott, Gregory A Hatley, Kate Moloney, Kristin Bohm, Maria J Gutierrez-Gines, Louise Weaver
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引用次数: 0

Abstract

Aims: Larvae of the black soldier fly (BSFL), Hermetia illucens, are recognized for their remarkable feeding flexibility and ability to convert a variety of organic waste streams into useful end products. Their ability to feed on both harmful and recalcitrant waste streams is thought to be due in part to plasticity in their gut microbiota, which shifts rapidly to select for taxa better suited to the incoming diet. Here, we aimed to exploit this feeding plasticity by using BSFL as "bioincubators," to grow and isolate plastic-degrading bacteria.

Methods and results: We fed larvae wheat bran containing a mix of microplastic (polyethylene terephthalate, polylactic acid, and polyhydroxybutyrate) and determined the community composition of plastic-degrading microorganisms using a combination of culturing techniques and next-generation sequencing. On average, more than a third of the gut microbiome was made up of genera that have previously demonstrated plastic degradation capabilities. To confirm this assessment, we isolated seven bacterial strains from plastic-fed BSFL that were positively identified as polyhydroxybutyrate-degraders in vitro.

Conclusions: Our results provide proof of concept that plastic-fed H. illucens could be used as bioincubators to grow plastic-degrading bacteria. We believe our methodology provides a simple model for verifying in silico results with in vitro tests and should be used to enhance the future isolation and characterization of novel plastic-degrading taxa.

研究塑料喂养的黑兵蝇幼虫(Hermetia illucens)作为塑料降解细菌的“生物孵化器”的潜力。
目的:黑兵蝇(Hermetia illucens)的幼虫以其卓越的觅食灵活性和将各种有机废物流转化为有用的最终产品的能力而闻名。它们以各种有害和顽固的废物流为食的能力被认为部分是由于它们肠道微生物群的可塑性,它们会迅速转移,选择更适合传入饮食的分类群。在这里,我们的目标是利用BSFL作为“生物孵化器”,培养和分离塑料降解细菌。方法和结果:我们用含有微塑料(聚对苯二甲酸乙二醇酯、聚乳酸和聚羟基丁酸酯)混合物的麦麸喂养幼虫,并使用培养技术和下一代测序相结合的方法确定了塑料降解微生物的群落组成。平均而言,超过三分之一的肠道微生物群是由以前已经显示出塑料降解能力的属组成的。为了证实这一评估,我们从塑料喂养的BSFL中分离出7株细菌菌株,这些菌株在体外被鉴定为聚羟基丁酸酯降解物。结论:我们的研究结果证明了塑料喂养的H. illucens可以作为生物孵化器来培养塑料降解细菌。我们相信我们的方法提供了一个简单的模型,可以通过体外测试验证计算机结果,并且应该用于加强未来新型塑料降解分类群的分离和表征。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Applied Microbiology
Journal of Applied Microbiology 生物-生物工程与应用微生物
CiteScore
7.30
自引率
2.50%
发文量
427
审稿时长
2.7 months
期刊介绍: Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.
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