{"title":"The long noncoding RNA CCDC144NL-AS1: prognosis significance and potential biological functions in cervical carcinoma.","authors":"Xuemei Hu, Yulong Wang, Hongyu Guo, Yuzhen Guo","doi":"10.1080/01443615.2025.2463416","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Recent research has shown that long noncoding RNA (lncRNA) performs a critical regulatory function across multiple malignancies. The involvement of RNA in the progression of multiple human cancer types has been elucidated; however, there is limited understanding regarding the importance of the lncRNA CCDC144NL-AS1 in cervical carcinoma (CC).</p><p><strong>Methods: </strong>CCDC144NL-AS1 expression was measured in 86 CC samples by RT-PCR. CC cell lines were engineered with CCDC144NL-AS1 overexpression using plasmid vectors, allowing for the exploration of its impact on the migration of tumour cells. Additionally, we studied how CCDC144NL-AS1 impacts epithelial-mesenchymal transition signalling, focusing on its expression levels and functional roles.</p><p><strong>Results: </strong>CCDC144NL-AS1 expression showed a notable increase in gynaecological tumour specimens and cell lines in contrast to normal control (all <i>p</i> < 0.05). This expression exhibited relationships with the clinical stage (<i>p</i> = 0.003), histologic grade (<i>p</i> = 0.020), and lymph node metastasis (<i>p</i> = 0.013). Additionally, survival rates were found to be lower in CC patients with increased CCDC144NL-AS1 expression, as confirmed by multivariate analysis, compared to individuals with reduced expression levels (Hazard ratio = 1.960; <i>p</i> = 0.020). CCDC144NL-AS1 also facilitated increased cell viability and migration while triggering the EMT pathway. Xenograft models demonstrated that lower CCDC144NL-AS1 levels resulted in smaller tumours and longer survival (median survival: 43.5 vs. 35.5 days, <i>p</i> < 0.05).</p><p><strong>Conclusion: </strong>CCDC144NL-AS1 significantly influences cell migration, making it a promising target for CC treatment and a valuable prognostic indicator.</p>","PeriodicalId":16627,"journal":{"name":"Journal of Obstetrics and Gynaecology","volume":"45 1","pages":"2463416"},"PeriodicalIF":0.9000,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Obstetrics and Gynaecology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/01443615.2025.2463416","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/4/8 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"OBSTETRICS & GYNECOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Recent research has shown that long noncoding RNA (lncRNA) performs a critical regulatory function across multiple malignancies. The involvement of RNA in the progression of multiple human cancer types has been elucidated; however, there is limited understanding regarding the importance of the lncRNA CCDC144NL-AS1 in cervical carcinoma (CC).
Methods: CCDC144NL-AS1 expression was measured in 86 CC samples by RT-PCR. CC cell lines were engineered with CCDC144NL-AS1 overexpression using plasmid vectors, allowing for the exploration of its impact on the migration of tumour cells. Additionally, we studied how CCDC144NL-AS1 impacts epithelial-mesenchymal transition signalling, focusing on its expression levels and functional roles.
Results: CCDC144NL-AS1 expression showed a notable increase in gynaecological tumour specimens and cell lines in contrast to normal control (all p < 0.05). This expression exhibited relationships with the clinical stage (p = 0.003), histologic grade (p = 0.020), and lymph node metastasis (p = 0.013). Additionally, survival rates were found to be lower in CC patients with increased CCDC144NL-AS1 expression, as confirmed by multivariate analysis, compared to individuals with reduced expression levels (Hazard ratio = 1.960; p = 0.020). CCDC144NL-AS1 also facilitated increased cell viability and migration while triggering the EMT pathway. Xenograft models demonstrated that lower CCDC144NL-AS1 levels resulted in smaller tumours and longer survival (median survival: 43.5 vs. 35.5 days, p < 0.05).
Conclusion: CCDC144NL-AS1 significantly influences cell migration, making it a promising target for CC treatment and a valuable prognostic indicator.
期刊介绍：
Journal of Obstetrics and Gynaecology represents an established forum for the entire field of obstetrics and gynaecology, publishing a broad range of original, peer-reviewed papers, from scientific and clinical research to reviews relevant to practice. It also includes occasional supplements on clinical symposia. The journal is read widely by trainees in our specialty and we acknowledge a major role in education in Obstetrics and Gynaecology. Past and present editors have recognized the difficulties that junior doctors encounter in achieving their first publications and spend time advising authors during their initial attempts at submission. The journal continues to attract a world-wide readership thanks to the emphasis on practical applicability and its excellent record of drawing on an international base of authors.
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