Immunological and histopathological changes in alkali burn-induced ocular surface pannus: Implication on success vs. failure of SLET

IF 3 2区医学 Q1 OPHTHALMOLOGY

Experimental eye research Pub Date : 2025-04-05 DOI:10.1016/j.exer.2025.110378

Abhinav Reddy Kethiri , Anahita Kate , Madhuri Amulya Koduri , Abhishek Sahoo , Harsha Agarwal , Tejaswini Pingali , Vijay Kumar Singh , Md Hasnat Ali , Dilip Kumar Mishra , Sayan Basu , Vivek Singh

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引用次数: 0

Abstract

Purpose

This study aimed to investigate the histopathological and immunological characteristics of human alkali burn ocular surface pannus in successful and failed cases of simple limbal epithelial transplantation (SLET).

Methods

Paraffin embedded pannus sections of limbal stem cell deficiency (LSCD) patients who underwent simple limbal epithelial transplantation were obtained from pathology. Samples included both cases of SLET failure (n = 12) and success (n = 7). Histological features were assessed using Hematoxylin-Eosin and Periodic acid–Schiff staining while infiltrated immune cells were characterised using immunohistochemistry. Data were represented as mean or median with the interquartile range and standard deviation. Multiple comparisons of means by linear mixed effect model with Tukey contrast and Bonferroni method were used.

Results

Conjunctivalization of the cornea that led to fibrous pannus formation was confirmed by the presence of CK19+ and CK3/12- cells. The median percentage of immune cell infiltrates like T-cells (CD3; success (9 %; IQR-9.2) vs. failure (23.5 %; IQR-10.2), (CD5; success (2.2 %; IQR-3) vs. failure (13.4 %; IQR-18.3), B-cells (CD20; success (3.2 %; IQR-3.2) vs. failure (4.1 %; IQR-5.5), antigen presenting cells (co-stimulatory CD40; success (7.8 %; IQR-8.9) vs. failure (16.4 %; IQR-7.1), and plasma cells (CD138; success (1.1 %; IQR-2.1) vs. failure (3.6 %; IQR-3.5) were identified in the pannus.

Conclusions

This study highlights a significantly higher infiltration of immune cells in the pannus and surrounding ocular tissue in cases of SLET failure compared to successful outcomes. These findings suggest that, strategies to reduce immune infiltration such as immunotherapy, corticosteroids, or amniotic membrane grafting should be considered prior to SLET to improve the success of limbal transplant surgery.

查看原文本刊更多论文

碱烧伤引起的眼表潘的免疫学和组织病理学变化：对SLET成功与失败的影响。

目的：探讨碱烧伤致角膜缘干细胞缺乏症（LSCD）患者眼表潘的组织病理学和免疫学特征。方法：对行单纯角膜缘上皮移植的LSCD患者病理切片进行石蜡包埋。样本包括眼表功能衰竭（n=12）和成功（n=7）两例。采用苏木精-伊红染色和周期性酸-希夫染色观察组织学特征，免疫组织化学染色观察浸润的免疫细胞。数据用四分位数范围或标准差表示为平均值或中位数。采用线性混合效应模型，采用Tukey对比法和Bonferroni法进行多重均值比较。结果：CK19+和CK3/12-细胞的存在证实了角膜结膜炎导致纤维膜的形成，而免疫细胞浸润如t细胞（CD3+）的中位数百分比(21.24%；Iqr-14.27), (cd5 +) (11.1%；IQR-9.74)， b细胞（CD20+） (4.28%；IQR-6.84)，浆细胞（CD138+） (3.67%；IQR-6.72)，抗原呈递细胞（共刺激标记- CD40+） (16.68%；在pannus中鉴定出IQR-10.3)。结论：本研究强调，与成功的结果相比，SLET失败的病例中，panus和周围眼组织中免疫细胞的浸润明显更高。这些发现表明，在SLET之前应考虑减少免疫浸润的策略，如免疫治疗、皮质类固醇或羊膜移植，以提高角膜缘移植手术的成功率。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Experimental eye research 医学-眼科学

CiteScore

6.80

自引率

5.90%

发文量

323

审稿时长

66 days

期刊介绍： The primary goal of Experimental Eye Research is to publish original research papers on all aspects of experimental biology of the eye and ocular tissues that seek to define the mechanisms of normal function and/or disease. Studies of ocular tissues that encompass the disciplines of cell biology, developmental biology, genetics, molecular biology, physiology, biochemistry, biophysics, immunology or microbiology are most welcomed. Manuscripts that are purely clinical or in a surgical area of ophthalmology are not appropriate for submission to Experimental Eye Research and if received will be returned without review.