Development and validation of simple colorimetric methods for assessing norfloxacin in pure form, in pharmaceutical products and in biological material.

IF 2.8 3区 医学 Q2 PHARMACOLOGY & PHARMACY
Muhammad Naeem Khan, Muhammad Adnan, Nusrat Bibi, Asif Kamal, Abd El-Zaher M A Mustafa, Iftikhar Ali
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Abstract

Two straightforward, affordable, accurate, and spectrophotometric techniques gross developed to assess norfloxacin, formulations, and biological samples. The oxidation of norfloxacin will be done in technique (A) in acid solution with help of Fe(III). A wavelength of 511 nm with a correlation coefficient of 0.9879 was produced by the resulting Fe(II) coupled with 1,10-phenanthroline and the red colour complex. A uniform absorbance ranging from 1 to 30 µg/mL was discovered. Similarly, in procedure (B), in an acidic medium, Ce(IV) was added to norfloxacin. After reacting with a specific amount of methyl orange, the residual Ce(IV) is then determined. An absorbance measurement at 508 nm and a correlation coefficient of 0.9966 indicate a straight-line relationship between the two variables for concentration range is 1-15 µg/mL. The procedures were developed after a careful analysis of the several elements that influence the reaction process. After calculations, the LOD (limits of detection) and LOQ (limit of quantification) were determined to be 1.098 and 1.111 µg/mL for method A and 2.875 and 3.368 µg/mL for method B respectively. The method B has also been applied for the determination of norfloxacin in spiked human plasma and urine samples. The percentage recoveries ranged from 98.74 to 103.43% and from 98.17 to 100.85% for plasma and urine samples, respectively. Proposed techniques have been successfully used for the examination of biological fluids, formulations for medicines as well as pure norfloxacin following statistical validation through recovery studies.

开发和验证用于评估纯形式、药品和生物材料中诺氟沙星的简单比色法。
开发了两种简单、经济、准确的分光光度法技术,用于评估诺氟沙星、制剂和生物样品。技术(A)在酸溶液中,利用铁(III)氧化诺氟沙星。得到的铁(II)与1,10-菲罗啉和红色配合物偶联的波长为511 nm,相关系数为0.9879。发现吸光度均匀,范围为1 ~ 30µg/mL。同样,在步骤(B)中,在酸性培养基中,将Ce(IV)添加到诺氟沙星中。与一定量的甲基橙反应后,测定残余Ce(IV)。在508 nm处测量吸光度,相关系数为0.9966,表明浓度范围为1 ~ 15µg/mL时,两个变量之间呈直线关系。这些程序是在仔细分析了影响反应过程的几个因素之后制定的。经计算,方法A的检出限和定量限分别为1.098和1.111µg/mL,方法B的检出限和定量限分别为2.875和3.368µg/mL。方法B也可用于加标人血浆和尿样中诺氟沙星的测定。血浆和尿液的加样回收率分别为98.74 ~ 103.43%和98.17 ~ 100.85%。建议的技术已成功地用于检查生物液体、药物配方以及通过回收率研究进行统计验证的纯诺氟沙星。
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来源期刊
BMC Pharmacology & Toxicology
BMC Pharmacology & Toxicology PHARMACOLOGY & PHARMACYTOXICOLOGY&nb-TOXICOLOGY
CiteScore
4.80
自引率
0.00%
发文量
87
审稿时长
12 weeks
期刊介绍: BMC Pharmacology and Toxicology is an open access, peer-reviewed journal that considers articles on all aspects of chemically defined therapeutic and toxic agents. The journal welcomes submissions from all fields of experimental and clinical pharmacology including clinical trials and toxicology.
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