C1QL1 regulates auditory nerve fibers growth via ELMO1-DOCK180-RAC1 integrin.

IF 1.2 4区医学 Q3 OTORHINOLARYNGOLOGY

Acta Oto-Laryngologica Pub Date : 2025-04-07 DOI:10.1080/00016489.2025.2486613

Minglin Chen, Yue Qi, Shusheng Gong

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引用次数: 0

Abstract

Background: Complement C1q Like 1 (C1QL1), a secreted protein, is known to play an important role in synaptic maturation, regulation, and maintenance in central nervous system. C1QL1 is expressed in adult cochlea inner and outer hair cells. However, the mechanism of C1QL1 in cochlea remains unclear.

Objective: This study aims to reveal the mechanism of C1ql1 gene in cochlea, including its target and pathway.

Material and methods: The protein-protein interaction analysis of C1QL1 was conducted with STRING database. The interaction between C1QL1 and BAI3 in cochlea was further confirmed by colocalization and co-immunoprecipitation. via upregulating and downregulating the expression of C1ql1, the factors in the pathway can be detected.

Results: C1QL1 protein interacts with BAI3 protein in cochlea. C1ql1 overexpression had no effect on auditory phenotype. C1ql1 regulates auditory nerve fibers growth via BAI3 and ELMO1-DOCK180-RAC1 integrin. Meanwhile, C1ql1 overexpression inhibited the expression of TIAM1-PARD3 integrin.

Conclusions and significance: C1QL1 interacts with BAI3 in cochlea and C1ql1 regulates auditory nerve fibers growth via ELMO1-DOCK180-RAC1 integrin. C1ql1 overexpression can inhibit the expression of TIAM1-PARD3 integrin.

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C1QL1通过ELMO1-DOCK180-RAC1整合素调控听神经纤维生长。

背景：补体C1q样1 （C1QL1）是一种分泌蛋白，在中枢神经系统突触成熟、调节和维持中起重要作用。C1QL1在成人耳蜗内、外毛细胞中表达。然而，C1QL1在耳蜗中的作用机制尚不清楚。目的：本研究旨在揭示C1ql1基因在耳蜗中的作用机制，包括其作用靶点和通路。材料与方法：使用STRING数据库进行C1QL1蛋白-蛋白互作分析。通过共定位和共免疫沉淀进一步证实了耳蜗中C1QL1和BAI3的相互作用。通过上调和下调C1ql1的表达，可以检测该通路的相关因子。结果：耳蜗中C1QL1蛋白与BAI3蛋白相互作用。C1ql1过表达对听觉表型无影响。C1ql1通过BAI3和ELMO1-DOCK180-RAC1整合素调控听神经纤维生长。同时，C1ql1过表达抑制TIAM1-PARD3整合素的表达。结论及意义：C1QL1与耳蜗中BAI3相互作用，C1QL1通过ELMO1-DOCK180-RAC1整合素调控听神经纤维生长。C1ql1过表达可抑制TIAM1-PARD3整合素的表达。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Oto-Laryngologica 医学-耳鼻喉科学

CiteScore

2.50

自引率

0.00%

发文量

审稿时长

3-6 weeks

期刊介绍： Acta Oto-Laryngologica is a truly international journal for translational otolaryngology and head- and neck surgery. The journal presents cutting-edge papers on clinical practice, clinical research and basic sciences. Acta also bridges the gap between clinical and basic research.