Natural biomass–derived carbon quantum dots: a path to antioxidant, anticancer, antibiofilm, and bacterial bioimaging potential

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2025-04-08 DOI:10.1007/s00604-025-07132-x

Prakashkumar Nallasamy, Pavithra Kannan, Manickam Selvaraj, Mohammed A. Assiri, Suganthy Natarajan

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引用次数: 0

Abstract

Eco-friendly, highly fluorescent, and biocompatible carbon quantum dots (CQDs) were synthesized from Typha angustifolia by the hydrothermal method. Fabricated CQDs were assessed for its bioimaging properties, anticancer potential, antibiofilm, and antioxidant activities. X-ray diffraction analysis indicates an amorphous nature, with an average particle size of 11 nm as observed in dynamic light scattering. TEM analysis revealed a uniform quasi-spherical-shaped structure. Photoluminescence studies reveal that CQDs exhibit an excitation at 390 nm with emissions at 484 nm and 474 nm. Water-soluble CQDs showed a potent antiproliferative effect against human breast cancer (MDA-MB-231) cell lines with an IC₅₀ value of 70.55 ± 0.015 µg/mL. Fluorescent studies revealed that CQDs enhanced the intracellular reactive oxygen species level leading to the loss of mitochondrial membrane potential triggering the apoptotic pathway culminating to cell death in MDA-MB-231 cells. In addition, CQDs showed a potent free radical scavenging activity with an IC₅₀ value of 21.1 ± 1.56 μg/mL exhibiting 94.69 ± 2.4% scavenging activity at the highest dose of 100 µg/mL. Furthermore, CQDs efficiently eradicated the biofilm-forming efficiency of Pseudomonas aeruginosa which frequently affects immunocompromised cancer patients. Cellular internalization studies in P. aeruginosa showed intense green fluorescence revealing the applicability of CQDs for bioimaging. Overall, the results indicate that CQDs fabricated from a natural source can serve as an excellent nanotheranostic and anti-infective agent for the treatment of triple-negative breast cancer.

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.