Lutein and Zeaxanthin abated neurobehavioral, neurochemical and oxido-inflammatory derangement in rats intoxicated with Aflatoxin B1

IF 2.6 4区医学 Q2 PHARMACOLOGY & PHARMACY

Toxicon Pub Date : 2025-04-01 DOI:10.1016/j.toxicon.2025.108345

Solomon Owumi , Joseph Chimezie , Marvellous O. Salami , Japheth A. Ishaya , Chidindu Vine Onyemuwa , Mark Nnamdi , Olatunde Owoeye

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引用次数: 0

Abstract

Aflatoxin B₁ (AFB₁), a mycotoxin commonly present in feed, has several toxic effects. AFB₁ seems to have a neurotoxic effect that leads to neurobehavioral impairment. On the other hand, Lutein and Zeaxanthin (LUT/ZEA) have antioxidant and anti-inflammatory effects. Here, we aimed to compare the effects of AFB₁ and the co-treatment with LUT/ZEA on neurobehavioural and biochemical changes viz-a-viz oxido-inflammatory response in male rats' hippocampal and pre-frontal cortexes. Experimental rats of the Wistar strain (n = 40) were randomly grouped into treatment cohorts: Control (corn oil 2 mL/kg), AFB₁ (75 μg/kg), LUT/ZEA only (100 mg/kg), AFB₁ + LUT/ZEA (75 μg/kg + 100 mg/kg), and AFB₁ + LUT/ZEA (75 μg/kg + 200 mg/kg). All groups were administered their respective treatment orally for 28 days, while behavioural tests were conducted using open field tests (OFT), Y-maze, novel object tests (NORT), and forced swim tests (FST) 1 h after treatment on day 26–28. The animals were euthanized on day 29. In the hippocampal and pre-frontal cortex, antioxidant indicators (SOD, CAT, GSH, GST, GPx, TSH), inflammatory mediators (XO, NO, MPO), and acetylcholinesterase activity were measured. Our finding presents the anti-oxidant effect of lutein/Zeaxanthin in the brains of AFB₁-intoxicated rats, indicating better cognitive and spatial memory capacity in Y-maze and NORT, an improvement in locomotive and explorative behaviour in OFT and reduction in anxio-depressive-like behaviour in LUT/ZEA co-treated rats. Acetylcholinesterase activity was enhanced in LUT/ZEA co-treated rats. LUT/ZEA co-treatment dampened oxido-inflammatory mediators by decreasing XO, NO, and MPO levels and increasing antioxidant activities (SOD, CAT, GSH, GST, GPx, TSH) in the prefrontal and hippocampal cortices. We surmise that mechanistically, co-treatment with LUT/ZEA effectively lessened AFB₁ neurotoxicity through anti-inflammatory and antioxidant pathways and essentially improved the experimental rats' neurobehavioural outcomes.

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叶黄素和玉米黄质可减轻黄曲霉毒素 B1 中毒大鼠的神经行为、神经化学和氧化炎症失调

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来源期刊

Toxicon 医学-毒理学

CiteScore

4.80

自引率

10.70%

发文量

358

审稿时长

68 days

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