Population data for 12 X-STR loci in the Orang Asli population of Peninsular Malaysia

IF 1.3 4区医学 Q3 MEDICINE, LEGAL

Legal Medicine Pub Date : 2025-04-05 DOI:10.1016/j.legalmed.2025.102615

Abdullah Nur Azeelah , Hanis Zainal Abidin NurWaliyuddin , Alwi Aedrianee Reeza , Zainuddin Zafarina

{"title":"Population data for 12 X-STR loci in the Orang Asli population of Peninsular Malaysia","authors":"Abdullah Nur Azeelah , Hanis Zainal Abidin NurWaliyuddin , Alwi Aedrianee Reeza , Zainuddin Zafarina","doi":"10.1016/j.legalmed.2025.102615","DOIUrl":null,"url":null,"abstract":"<div><div>The Orang Asli, the earliest inhabitants of Peninsular Malaysia, constitute only 0.6% of the country’s population. This study analyses allele frequencies and forensic parameters for 12 X-STR loci across six Orang Asli subgroups using the Investigator® Argus X-12 QS kit. Blood samples were collected from 164 individuals (68 males, 96 females), with genomic DNA extracted and analysed through PCR amplification, capillary electrophoresis, and allele assignment. A total of 117 alleles were identified, with DXS10135 being the most polymorphic and DXS8378 the least. The highest power of discrimination was observed at DXS10135 for both males and females. The highest haplotype diversity was found in linkage group 4 with 39 haplotypes, while linkage group 3 had the lowest with 23 haplotypes. Forensic statistical analysis confirmed a combined power of discrimination and mean exclusion chance exceeding 0.999999. These findings provide valuable data for forensic applications, population genetics, and anthropological research.</div></div>","PeriodicalId":49913,"journal":{"name":"Legal Medicine","volume":"75 ","pages":"Article 102615"},"PeriodicalIF":1.3000,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Legal Medicine","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1344622325000495","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MEDICINE, LEGAL","Score":null,"Total":0}

引用次数: 0

Abstract

The Orang Asli, the earliest inhabitants of Peninsular Malaysia, constitute only 0.6% of the country’s population. This study analyses allele frequencies and forensic parameters for 12 X-STR loci across six Orang Asli subgroups using the Investigator® Argus X-12 QS kit. Blood samples were collected from 164 individuals (68 males, 96 females), with genomic DNA extracted and analysed through PCR amplification, capillary electrophoresis, and allele assignment. A total of 117 alleles were identified, with DXS10135 being the most polymorphic and DXS8378 the least. The highest power of discrimination was observed at DXS10135 for both males and females. The highest haplotype diversity was found in linkage group 4 with 39 haplotypes, while linkage group 3 had the lowest with 23 haplotypes. Forensic statistical analysis confirmed a combined power of discrimination and mean exclusion chance exceeding 0.999999. These findings provide valuable data for forensic applications, population genetics, and anthropological research.

查看原文本刊更多论文

马来西亚半岛奥朗阿斯利人 12 个 X-STR 位点的人口数据

原住民是马来西亚半岛最早的居民，只占全国人口的0.6%。本研究使用调查员®Argus X-12 QS试剂盒分析了6个猩猩亚群中12个X-STR基因座的等位基因频率和法医参数。采集164例个体（男性68例，女性96例）的血样，提取基因组DNA，并通过PCR扩增、毛细管电泳和等位基因分配进行分析。共鉴定出117个等位基因，其中DXS10135多态性最多，DXS8378多态性最少。在DXS10135中，男性和女性的歧视率最高。单倍型多样性最高的连锁组4有39个单倍型，最低的连锁组3有23个单倍型。法医学统计分析证实，辨别力和平均排除机会的总和超过0.999999。这些发现为法医应用、群体遗传学和人类学研究提供了有价值的数据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Legal Medicine Nursing-Issues, Ethics and Legal Aspects

CiteScore

2.80

自引率

6.70%

发文量

119

审稿时长

7.9 weeks

期刊介绍： Legal Medicine provides an international forum for the publication of original articles, reviews and correspondence on subjects that cover practical and theoretical areas of interest relating to the wide range of legal medicine. Subjects covered include forensic pathology, toxicology, odontology, anthropology, criminalistics, immunochemistry, hemogenetics and forensic aspects of biological science with emphasis on DNA analysis and molecular biology. Submissions dealing with medicolegal problems such as malpractice, insurance, child abuse or ethics in medical practice are also acceptable.