Octacosanol ameliorates oligozoospermia in mice through improving cell cycle arrest and regulating testosterone/androgen receptor pathway

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods Pub Date : 2025-04-09 DOI:10.1016/j.jff.2025.106811

Taowen Ye , Xiaoran Li , Weizhen Wang , Mingyu Jiang , Fanshuo Zeng , Zihan Zheng , Jianwu Shi , Lei Zhang , Chengniu Wang

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Abstract

Octacosanol (OCT), a nutritional supplement with well-documented biological properties, has not been thoroughly investigated for its impact on oligozoospermia (Oligo) and its molecular mechanisms. In this study, the Oligo mouse model was established by intraperitoneal injection of busulfan. The ICR male mice were randomly divided into Normal, Oligo, and Oligo + OCT (5, 10, or 20 mg/kg) groups, with the highest concentration of OCT showing no organ toxicity. OCT was found to reverse busulfan-induced testicular damage, testicular cytoskeletal impairment and sperm count reduction, while alleviating oxidative stress in Oligo mice. RNA sequencing revealed significant gene expression differences related to ‘nuclear division’ and ‘cell cycle’ between the Oligo and OCT-treated groups. OCT also elevated serum hormone levels in Oligo mice. Western Blot and immunofluorescence confirmed that OCT enhances spermatogenesis through improving cell cycle arrest and regulating testosterone/androgen receptor pathway. OCT may serve as a potential therapeutic agent for male infertility.

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八糖醇通过改善细胞周期阻滞和调节睾酮/雄激素受体通路改善小鼠少精症

八烷糖醇（OCT）是一种营养补充剂，具有良好的生物学特性，但其对少精症（Oligo）的影响及其分子机制尚未得到充分的研究。本研究采用丁硫凡腹腔注射法建立Oligo小鼠模型。将ICR雄性小鼠随机分为Normal组、Oligo组和Oligo + OCT组（5、10、20 mg/kg），最高OCT浓度无器官毒性。在Oligo小鼠中，OCT可以逆转丁硫芬诱导的睾丸损伤、睾丸细胞骨架损伤和精子数量减少，同时减轻氧化应激。RNA测序显示，Oligo和oct处理组之间与“核分裂”和“细胞周期”相关的基因表达存在显著差异。OCT还能提高Oligo小鼠的血清激素水平。Western Blot和免疫荧光证实OCT通过改善细胞周期阻滞和调节睾酮/雄激素受体通路促进精子发生。OCT可能作为男性不育症的潜在治疗剂。

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来源期刊

Journal of Functional Foods FOOD SCIENCE & TECHNOLOGY-

CiteScore

9.60

自引率

1.80%

发文量

428

审稿时长

76 days

期刊介绍： Journal of Functional Foods continues with the same aims and scope, editorial team, submission system and rigorous peer review. We give authors the possibility to publish their top-quality papers in a well-established leading journal in the food and nutrition fields. The Journal will keep its rigorous criteria to screen high impact research addressing relevant scientific topics and performed by sound methodologies. The Journal of Functional Foods aims to bring together the results of fundamental and applied research into healthy foods and biologically active food ingredients. The Journal is centered in the specific area at the boundaries among food technology, nutrition and health welcoming papers having a good interdisciplinary approach. The Journal will cover the fields of plant bioactives; dietary fibre, probiotics; functional lipids; bioactive peptides; vitamins, minerals and botanicals and other dietary supplements. Nutritional and technological aspects related to the development of functional foods and beverages are of core interest to the journal. Experimental works dealing with food digestion, bioavailability of food bioactives and on the mechanisms by which foods and their components are able to modulate physiological parameters connected with disease prevention are of particular interest as well as those dealing with personalized nutrition and nutritional needs in pathological subjects.