Dual-Bessel-Beam Stimulated Emission Depletion Microscopy for Super-resolution Volumetric Projection Imaging of Lipid Droplet Dynamics

IF 9.1 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Nano Letters Pub Date : 2025-03-17 DOI:10.1021/acs.nanolett.4c0486710.1021/acs.nanolett.4c04867

Renlong Zhang, Haoxian Zhou, Chenguang Wang, Xiaoyu Weng, Liwei Liu, Peng Xi* and Junle Qu*,

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Abstract

Volumetric imaging efficiently captures comprehensive spatial structures and dynamic function information on organisms in biomedical research. However, optical diffraction limit restricts the visualization of fine structure details at nanoscale. To address this limitation, we developed dual-Bessel-beam stimulated emission depletion (DB-STED) microscopy to enhance the information throughput and lateral resolution. This technique combines a zeroth-order Bessel beam for excitation with a first-order hollow Bessel beam for depletion, aligned both spatially and temporally to achieve super-resolution volumetric projection imaging. We validated this approach using fluorescent beads embedded in agarose, achieving a resolution of 69 nm over a depth of 10 μm with a numerical aperture of 1.4. The high-throughput and super-resolution capability enables detailed observation of lipid droplet motion within entire cells, providing valuable insights into lipid dynamics.

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用于脂滴动态超分辨率体积投影成像的双贝塞尔光束受激发射损耗显微镜

在生物医学研究中，体积成像技术能有效地捕获生物的空间结构和动态功能信息。然而，光学衍射极限限制了纳米尺度下精细结构细节的可视化。为了解决这一限制，我们开发了双贝塞尔光束激发发射耗尽（DB-STED）显微镜，以提高信息吞吐量和横向分辨率。该技术结合了用于激发的零阶贝塞尔光束和用于耗尽的一阶空心贝塞尔光束，在空间和时间上对齐，以实现超分辨率的体投影成像。我们用琼脂糖包埋的荧光珠验证了这种方法，在10 μm的深度上获得了69 nm的分辨率，数值孔径为1.4。高通量和超分辨率的能力可以详细观察整个细胞内的脂滴运动，为脂质动力学提供有价值的见解。

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来源期刊

Nano Letters 工程技术-材料科学：综合

CiteScore

16.80

自引率

2.80%

发文量

1182

审稿时长

1.4 months

期刊介绍： Nano Letters serves as a dynamic platform for promptly disseminating original results in fundamental, applied, and emerging research across all facets of nanoscience and nanotechnology. A pivotal criterion for inclusion within Nano Letters is the convergence of at least two different areas or disciplines, ensuring a rich interdisciplinary scope. The journal is dedicated to fostering exploration in diverse areas, including: - Experimental and theoretical findings on physical, chemical, and biological phenomena at the nanoscale - Synthesis, characterization, and processing of organic, inorganic, polymer, and hybrid nanomaterials through physical, chemical, and biological methodologies - Modeling and simulation of synthetic, assembly, and interaction processes - Realization of integrated nanostructures and nano-engineered devices exhibiting advanced performance - Applications of nanoscale materials in living and environmental systems Nano Letters is committed to advancing and showcasing groundbreaking research that intersects various domains, fostering innovation and collaboration in the ever-evolving field of nanoscience and nanotechnology.