TaDTGIP1-TaDTG6-BDel574-TaPIF1 module regulates drought stress response in wheat

IF 8.3 1区生物学 Q1 PLANT SCIENCES

New Phytologist Pub Date : 2025-04-07 DOI:10.1111/nph.70123

Linying Du, Qiannan Wang, Li Ding, Fangfang Li, Chunhao Fang, Hanxiao Qu, Chen Wang, Ping Jiang, Bin Chen, Zhen Qin, Zhensheng Kang, Hude Mao

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引用次数: 0

Abstract

Drought is a major environmental constraint to wheat production, yet the genetic and molecular mechanisms underlying drought tolerance remain poorly understood. A gain-of-function protein variant TaDTG6-B^Del574 has been identified and positively regulates TaPIF1 transcription to enhance wheat drought tolerance. However, the precise molecular pathways driving this response are yet to be fully characterized.
In this study, we demonstrate that TaPIF1 plays a crucial role in mediating wheat drought tolerance by regulating stomatal aperture to control transpiration. RNA sequencing combined with biochemical assays revealed that TaPIF1 directly binds to E-box elements to activate the expression of key stress-responsive genes, including TaABI5, TaRD17, and TaP5CS1. Notably, overexpression of TaABI5 enhances wheat drought tolerance by promoting stomatal closure, thereby reducing water loss.
Furthermore, TaPIF1 interacts with TaABI5 and the bHLH transcription factor TaAKS1 to synergistically enhancing the transcriptional activation of TaABI5, TaRD17, and TaP5CS1. Additionally, our findings verified that TaDTGIP1 interacts with TaDTG6-B^Del574 to attenuate its binding affinity and regulatory activity on the TaPIF1 promoter, thereby negatively regulating drought tolerance.
Together, our findings unveil the molecular mechanisms underlying wheat drought stress response mediated by the TaDTGIP1-TaDTG6-B^Del574-TaPIF1/TaABI5/TaAKS1-target regulatory module and identify potential candidate genes for breeding elite drought-tolerant wheat varieties.

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.