Automated Red and White Blood Cell Counting in Capillary Microsamples by Sysmex-XN Predilution Mode

IF 2.3 4区医学 Q3 HEMATOLOGY

International Journal of Laboratory Hematology Pub Date : 2025-04-06 DOI:10.1111/ijlh.14478

Claus Vinter Bødker Hviid, Vibeke Staun Christensen, Klaus Rosenkilde Jensen, Julia Roman Møller, Anja Reinert Hansen

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引用次数: 0

Abstract

Objectives

Neonatal patients present a challenge to the clinical laboratory because of their low blood volume. The Sysmex XN-series features a predilution (PD) mode allowing hematologic measurements with only 20–50 μL of blood. We verified the PD mode for analysis of selected hematologic parameters in 50 μL microsamples.

Methods

Microsamples were prepared from adult EDTA blood. White blood cell count (leukocytes, neutrophils, and lymphocytes) and red blood cell parameters (erythrocytes, hemoglobin, hematocrit, mean cell volume, and reticulocytes) were evaluated. The imprecision of the PD mode was evaluated over 3 days, and the accuracy was assessed by method comparison with the standard whole blood mode. The effect of capillary sampling, microsample stability during storage, and pneumatic tube transportation was evaluated. Finally, the bias was reproduced in a small sample of pediatric patients.

Results

For white blood cells, the bias was ≤ 5.4% (95% CI: 4.5–6.2) and imprecision was below 3.5% (except at the lowest levels). Capillary sampling had little effect on PD analytical performance (bias ≤ 0.7% and imprecision ≤ 4.7%) and white blood cell counts were stable for 7 h at room temperature and after pneumatic tube transportation. The red blood cell parameters generally exceeded the allowable bias and imprecision. The bias of the pediatric samples remained within the 95% PI for the method comparison studies.

Conclusion

The PD mode has acceptable analytical performance and preanalytical stability for white blood cell counts but not for red blood cell parameters. It may offer a low-volume alternative for hematologic monitoring.

Abstract Image

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Sysmex-XN预稀释模式在毛细管微样品中的自动红细胞和白细胞计数。

目的：新生儿的低血容量给临床实验室带来了挑战。Sysmex xn系列具有预稀释（PD）模式，仅允许20-50 μL血液进行血液学测量。我们验证了PD模式用于分析50 μL微样品中选定的血液学参数。方法：制备成人EDTA血显微标本。评估白细胞计数（白细胞、中性粒细胞和淋巴细胞）和红细胞参数（红细胞、血红蛋白、红细胞比容、平均细胞体积和网织红细胞）。在3天内评估PD模式的不精确性，并通过与标准全血模式的方法比较来评估准确性。评估了毛细管取样、微样品储存稳定性和气动管道运输的影响。最后，在儿科患者的小样本中重现了偏倚。结果：对于白细胞，偏差≤5.4% (95% CI: 4.5-6.2)，不精确性低于3.5%（最低水平除外）。毛细管取样对PD分析性能影响不大（偏差≤0.7%，不精度≤4.7%），白细胞计数在室温和气动输送后7 h内保持稳定。红细胞参数一般超过允许的偏差和不精度。在方法比较研究中，儿童样本的偏倚保持在95% PI范围内。结论：PD模式对白细胞计数具有良好的分析性能和分析前稳定性，但对红细胞参数不具有良好的分析性能。它可能为血液学监测提供一个小容量的替代方案。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Laboratory Hematology 医学-血液学

CiteScore

4.50

自引率

6.70%

发文量

211

审稿时长

6-12 weeks

期刊介绍： The International Journal of Laboratory Hematology provides a forum for the communication of new developments, research topics and the practice of laboratory haematology. The journal publishes invited reviews, full length original articles, and correspondence. The International Journal of Laboratory Hematology is the official journal of the International Society for Laboratory Hematology, which addresses the following sub-disciplines: cellular analysis, flow cytometry, haemostasis and thrombosis, molecular diagnostics, haematology informatics, haemoglobinopathies, point of care testing, standards and guidelines. The journal was launched in 2006 as the successor to Clinical and Laboratory Hematology, which was first published in 1979. An active and positive editorial policy ensures that work of a high scientific standard is reported, in order to bridge the gap between practical and academic aspects of laboratory haematology.