Aloe Vera Gel and Rind-Derived Nanoparticles Mitigate Skin Photoaging via Activation of Nrf2/ARE Pathway.

IF 6.6 2区医学 Q1 NANOSCIENCE & NANOTECHNOLOGY

International Journal of Nanomedicine Pub Date : 2025-04-02 eCollection Date: 2025-01-01 DOI:10.2147/IJN.S510352

Zixuan Sun, Yuzhou Zheng, Tangrong Wang, Jiaxin Zhang, Jiali Li, Zhijing Wu, Fan Zhang, Tingxin Gao, Li Yu, XueZhong Xu, Hui Qian, Yulin Tan

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引用次数: 0

Abstract

Background: Skin aging is the primary external manifestation of human aging, and long-term exposure to ultraviolet radiation is the leading cause of photoaging, which can lead to actinic keratosis and skin cancer in severe cases. Traditional treatments may pose safety risks and cause side effects. As an emerging research direction, plant-derived exosome-like nanoparticles (PDNPs) show promise in combating aging. Aloe vera, known for its natural active ingredients that benefit the skin, aloe-derived exosome-like nanoparticles (ADNPs) have not yet been studied for their potential in delaying skin aging.

Methods: In this study, nanoparticles were isolated from two different sites, aloe vera gel and aloe vera rind (gADNPs and rADNPs), and characterized by TEM, SEM, AFM, NTA and BCA. The effects were evaluated by constructing in vitro and in vivo models and using RT-qPCR, immunofluorescence, and histopathological analysis.

Results: The results first revealed the exceptional anti-aging effects of ADNPs. We found that ADNPs promoted the nuclear translocation of Nrf2, alleviated oxidative stress and DNA damage induced by UV exposure, and inhibited the elevation of β-gal and SASP. In vivo, ADNPs reduced MDA and SOD levels in mouse skin tissue and delayed skin photoaging. Moreover, safety assessments confirmed the excellent biocompatibility of ADNPs.

Conclusion: ADNPs delay skin photoaging through the Nrf2/ARE pathway, holding potential clinical application value, and may provide new therapeutic strategies for future medical cosmetology and skin disease prevention.

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芦荟凝胶和果皮衍生纳米颗粒通过激活Nrf2/ARE通路减缓皮肤光老化。

背景：皮肤老化是人类衰老的主要外在表现，长期暴露在紫外线下是光老化的主要原因，光老化可导致光化性角化病，严重者可导致皮肤癌。传统的治疗方法可能存在安全风险和副作用。作为一个新兴的研究方向，植物源性外泌体样纳米颗粒（PDNPs）在抗衰老方面具有广阔的前景。芦荟以其有益于皮肤的天然活性成分而闻名，但芦荟衍生的外泌体样纳米颗粒（ADNPs）在延缓皮肤衰老方面的潜力尚未得到研究。方法：从芦荟凝胶和芦荟皮两个不同部位（gADNPs和rADNPs）分离纳米颗粒，并通过TEM、SEM、AFM、NTA和BCA进行表征。通过构建体外和体内模型、RT-qPCR、免疫荧光和组织病理学分析来评估其效果。结果：首次揭示了ADNPs独特的抗衰老作用。我们发现ADNPs促进Nrf2的核易位，减轻紫外线照射引起的氧化应激和DNA损伤，抑制β-gal和SASP的升高。在体内，ADNPs降低小鼠皮肤组织中MDA和SOD水平，延缓皮肤光老化。此外，安全性评估证实了ADNPs具有良好的生物相容性。结论：ADNPs通过Nrf2/ARE途径延缓皮肤光老化，具有潜在的临床应用价值，可能为未来医学美容和皮肤病预防提供新的治疗策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Nanomedicine NANOSCIENCE & NANOTECHNOLOGY-PHARMACOLOGY & PHARMACY

CiteScore

14.40

自引率

3.80%

发文量

511

审稿时长

1.4 months

期刊介绍： The International Journal of Nanomedicine is a globally recognized journal that focuses on the applications of nanotechnology in the biomedical field. It is a peer-reviewed and open-access publication that covers diverse aspects of this rapidly evolving research area. With its strong emphasis on the clinical potential of nanoparticles in disease diagnostics, prevention, and treatment, the journal aims to showcase cutting-edge research and development in the field. Starting from now, the International Journal of Nanomedicine will not accept meta-analyses for publication.