{"title":"The antibiofilm potential of Clidemia hirta against Candida albicans: preliminary study.","authors":"Sufi Desrini","doi":"10.1186/s13104-025-07209-z","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>This study aims to screen the activity of ethanol extract of Clidemia hirta fruits and leaves against 24 h old Candida albicans biofilm by measuring cell viability with 3-(4,5-dimethylthiazol- 2-yl)- 2,5-diphenyl tetrazolium bromide (MTT) and planktonic cells of C. albicans using the broth microdilution method.</p><p><strong>Results: </strong>The ethanol extract of C. hirta fruits inhibited (50%) the C. albicans biofilm at a concentration fourfold higher than those required to inhibit planktonic cells (31.25 µg/mL vs. 7.8 µg/mL). This finding is supported by the morphological changes of C. albicans biofilm under scanning electron microscopy (SEM) visualization. A total of 55 compounds were detected using UHPLC coupled with Thermo Scientific™ Q Exactive™ High-Resolution Mass Spectrometer (HRMS). The top three compounds of the ethanol extract: dodecyl sulfate, bis(2-ethylhexyl) phthalate, and erucamide) might be responsible compounds for the antibiofilm activity of ethanol extract of C. hirta.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"143"},"PeriodicalIF":1.6000,"publicationDate":"2025-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11972527/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"BMC Research Notes","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1186/s13104-025-07209-z","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: This study aims to screen the activity of ethanol extract of Clidemia hirta fruits and leaves against 24 h old Candida albicans biofilm by measuring cell viability with 3-(4,5-dimethylthiazol- 2-yl)- 2,5-diphenyl tetrazolium bromide (MTT) and planktonic cells of C. albicans using the broth microdilution method.
Results: The ethanol extract of C. hirta fruits inhibited (50%) the C. albicans biofilm at a concentration fourfold higher than those required to inhibit planktonic cells (31.25 µg/mL vs. 7.8 µg/mL). This finding is supported by the morphological changes of C. albicans biofilm under scanning electron microscopy (SEM) visualization. A total of 55 compounds were detected using UHPLC coupled with Thermo Scientific™ Q Exactive™ High-Resolution Mass Spectrometer (HRMS). The top three compounds of the ethanol extract: dodecyl sulfate, bis(2-ethylhexyl) phthalate, and erucamide) might be responsible compounds for the antibiofilm activity of ethanol extract of C. hirta.
BMC Research NotesBiochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
3.60
自引率
0.00%
发文量
363
审稿时长
15 weeks
期刊介绍：
BMC Research Notes publishes scientifically valid research outputs that cannot be considered as full research or methodology articles. We support the research community across all scientific and clinical disciplines by providing an open access forum for sharing data and useful information; this includes, but is not limited to, updates to previous work, additions to established methods, short publications, null results, research proposals and data management plans.
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