Gut microbiome in patients with early-stage and late-stage endometriosis.

IF 2.4 3区 医学 Q2 OBSTETRICS & GYNECOLOGY
Zhaoxia Cai, Ziwei Zhou, Sixia Huang, Song Ma, Yuying Chen, Yuzhen Cao, Ying Ma
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引用次数: 0

Abstract

Background: Endometriosis is a chronic inflammatory gynecological disease. Previous studies have explored relationships between endometriosis and the microbiota, but none have focused on differences in gut microbiota between early-stage and late-stage endometriosis patients or their connections to dysmenorrhea symptoms. This study compared gut microbiota compositions between early-stage and late-stage endometriosis patients using amplicon sequencing and further analyzed their dysmenorrhea symptoms.

Methods: To minimize seasonal and dietary impacts, we recruited Guangdong residents hospitalized for surgery at Zhujiang Hospital. Participants underwent preoperative screening based on enrollment criteria and fecal samples were collected. Endometriosis was classified according to the American Society for Reproductive Medicine (ASRM) staging system based on surgincal and pathological findings. Stage I-II cases were designated as early-stage endometriosis, and Stage III-IV as late-stage endometriosis.

Results: A total of 112 patient fecal samples were collected, with 75 (median age, 32 years [range, 18-49 years]) meeting the enrollment criteria, including 39 early-stage (32 Stage I and 7 Stage II) and 36 late-stage (16 Stage III and 20 Stage IV) patients. The gut microbiota structure and functions in early-stage patients significantly differed from those in late-stage cases. Dysmenorrhea was associated with specific microbial traits. Late-stage patients with dysmenorrhea displayed distinctly different gut profiles compared to other endometriosis groups. Bartonella, Snodgrassella, and other taxa were enriched in late-stage cases, while Bacteroides, and Prevotella were decreased.

Conclusion: The gut microbial community structure in early-stage endometriosis patients significantly differs from that in late-stage cases, with late-stage patients experiencing dysmenorrhea displaying particularly distinct gut profiles. Predicted functional analysis indicated suppressed steroid biosynthesis pathways in the gut of late-stage endometriosis patients. In conclusion, it is plausible that the multiple effects of steroids on the lower gastrointestinal tract may involve microbiota alterations, suggesting the need for further investigations.

早期和晚期子宫内膜异位症患者的肠道微生物组。
背景:子宫内膜异位症是一种慢性炎症性妇科疾病。以前的研究已经探讨了子宫内膜异位症与微生物群之间的关系,但没有研究关注早期和晚期子宫内膜异位症患者肠道微生物群的差异或它们与痛经症状的联系。本研究利用扩增子测序比较了早期和晚期子宫内膜异位症患者的肠道菌群组成,并进一步分析了他们的痛经症状。方法:为了尽量减少季节和饮食的影响,我们招募了在珠江医院外科住院的广东省居民。参与者根据入组标准进行术前筛查,并收集粪便样本。子宫内膜异位症根据美国生殖医学学会(ASRM)基于手术和病理结果的分期系统进行分类。I-II期为早期子宫内膜异位症,III-IV期为晚期子宫内膜异位症。结果:共收集患者粪便样本112例,符合入选标准的患者75例(年龄中位数32岁[范围18-49岁]),其中早期39例(I期32例,II期7例),晚期36例(III期16例,IV期20例)。早期患者的肠道菌群结构和功能与晚期患者有显著差异。痛经与特定的微生物性状有关。与其他子宫内膜异位症组相比,晚期痛经患者表现出明显不同的肠道特征。晚期病例Bartonella、Snodgrassella等类群丰富,Bacteroides、Prevotella减少。结论:早期子宫内膜异位症患者的肠道微生物群落结构与晚期患者存在显著差异,晚期痛经患者的肠道菌群特征尤为明显。预测功能分析表明,在晚期子宫内膜异位症患者的肠道抑制类固醇生物合成途径。总之,类固醇对下胃肠道的多重作用可能涉及微生物群的改变,这表明需要进一步的研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
BMC Women's Health
BMC Women's Health OBSTETRICS & GYNECOLOGY-
CiteScore
3.40
自引率
4.00%
发文量
444
审稿时长
>12 weeks
期刊介绍: BMC Women''s Health is an open access, peer-reviewed journal that considers articles on all aspects of the health and wellbeing of adolescent girls and women, with a particular focus on the physical, mental, and emotional health of women in developed and developing nations. The journal welcomes submissions on women''s public health issues, health behaviours, breast cancer, gynecological diseases, mental health and health promotion.
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