Serum and urine metabolite correlates of vitamin D supplementation in the Atherosclerosis Risk in Communities (ARIC) study

IF 2.9 Q3 NUTRITION & DIETETICS

Clinical nutrition ESPEN Pub Date : 2025-04-04 DOI:10.1016/j.clnesp.2025.03.172

Valerie K. Sullivan , Jingsha Chen , Lauren Bernard , Bing Yu , Erin D. Michos , Lawrence J. Appel , Alice H. Lichtenstein , Casey M. Rebholz

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引用次数: 0

Abstract

Background & aims

Vitamin D regulates calcium and phosphorus homeostasis, skeletal health, and potentially other aspects of health. There are limitations of existing vitamin D biomarkers. We aimed to discover novel vitamin D biomarkers by investigating serum and urine metabolites associated with vitamin D supplementation.

Methods

We examined cross-sectional associations between vitamin D supplementation and serum and urine metabolites in Atherosclerosis Risk in Communities Study participants at visit 5 (2011–2013). Untargeted metabolomic profiling of serum and spot urine samples was performed by Metabolon, Inc. We analyzed associations between vitamin D supplementation and log₂-transformed metabolites using linear regression models adjusted for demographic, lifestyle, and health covariates.

Results

Of 5225 participants with serum metabolites analyzed (mean age 76 [SD 5] years, 57 % female, 20 % Black), 45 % reported taking vitamin D supplements. Eighty-two of 933 serum metabolites were associated with vitamin D supplementation (P < 0.05/933). Most were lipids (n = 36). Of 1565 participants with urine metabolites analyzed, one-third (37 %) used vitamin D. Nineteen of 946 urine metabolites were associated with vitamin D supplementation (P < 0.05/946). Most were cofactors and vitamins (n = 12). After adjusting for other supplement use (multivitamin/mineral, omega-3, B and C vitamins), 5 serum metabolites (pro-hydroxy-pro, pyroglutamine, sulfate, creatine, and 2-hydroxypalmitate) and no urine metabolites were significantly associated with vitamin D supplementation.

Conclusions

Many serum and urine metabolites were associated with vitamin D supplementation. Five serum metabolites remained associated with vitamin D after adjustment for other dietary supplements, including metabolites of bone collagen degradation, glutathione metabolism, and sphingolipid metabolism. These metabolites may reflect physiological activities of vitamin D and, thus, improve assessment of vitamin D adequacy to achieve functional outcomes. These merit further investigation as potential vitamin D biomarkers.

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血清和尿液代谢物与社区动脉粥样硬化风险（ARIC）研究中维生素D补充的相关性

背景与目的：维生素D调节钙和磷的体内平衡，骨骼健康，以及潜在的其他健康方面。现有的维生素D生物标志物存在局限性。我们旨在通过研究与维生素D补充相关的血清和尿液代谢物来发现新的维生素D生物标志物。方法：我们在访问5（2011-2013）时检查了维生素D补充与社区研究参与者动脉粥样硬化风险中血清和尿液代谢物之间的横断面关联。血清和尿样的非靶向代谢组学分析由Metabolon， Inc.进行。我们使用线性回归模型分析了维生素D补充与log2转化代谢物之间的关系，并对人口统计学、生活方式和健康协变量进行了调整。结果：在5225名血清代谢物分析参与者中（平均年龄76岁[SD 5]岁，57%为女性，20%为黑人），45%报告服用维生素D补充剂。933例血清代谢物中有82例与补充维生素D有关。结论：许多血清和尿液代谢物与补充维生素D有关。在调整其他膳食补充剂后，仍有5种血清代谢物与维生素D相关，包括骨胶原降解代谢物、谷胱甘肽代谢和鞘脂代谢。这些代谢物可能反映了维生素D的生理活动，从而改善了对维生素D充分性的评估，以达到功能性结果。这些作为潜在的维生素D生物标志物值得进一步研究。

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来源期刊

Clinical nutrition ESPEN NUTRITION & DIETETICS-

CiteScore

4.90

自引率

3.30%

发文量

512

期刊介绍： Clinical Nutrition ESPEN is an electronic-only journal and is an official publication of the European Society for Clinical Nutrition and Metabolism (ESPEN). Nutrition and nutritional care have gained wide clinical and scientific interest during the past decades. The increasing knowledge of metabolic disturbances and nutritional assessment in chronic and acute diseases has stimulated rapid advances in design, development and clinical application of nutritional support. The aims of ESPEN are to encourage the rapid diffusion of knowledge and its application in the field of clinical nutrition and metabolism. Published bimonthly, Clinical Nutrition ESPEN focuses on publishing articles on the relationship between nutrition and disease in the setting of basic science and clinical practice. Clinical Nutrition ESPEN is available to all members of ESPEN and to all subscribers of Clinical Nutrition.