Rubrolithibacter danxiaensis gen. nov., sp. nov. isolated from red sandy conglomerate in the Danxia Mountain

Abstract

A Gram-stain-negative, rod-shaped and non-motile bacterium, designated as strain SYSU DXS3258^T, was isolated from red sandy conglomerate sample collected from the Danxia Mountain in Guangdong Province, PR China. The bacterium was found to grow optimally at 28 °C, pH 7.0, and with 0–1% (w/v) NaCl. Phylogenetic analyses based on the 16S rRNA gene sequences placed strain SYSU DXS3258^T within the family Sphingobacteriaceae. Strain SYSU DXS3258^T showed the highest 16S rRNA gene sequence similarity to Pararcticibacter tournemirensis TF5-37.2-LB10^T (95.5%), Paradesertivirga mongoliensis 1-32 ^T (95.3%) and Desertivirga xinjiangensis 12157 ^T (95.2%). Digital DNA–DNA hybridization, orthologous average nucleotide identity and average amino acid identity values between strain SYSU DXS3258^T and the closest related members within the Sphingobacteriaceae were ≤ 13%, 70.3% and 69.9%, respectively. The genomic DNA G + C content was 38.8%. The major fatty acid was iso-C_15:0, C_18:0 and C_15:0 2-OH. The predominant polar lipids were phosphatidylethanolamine, three glycolipids, one phospholipid and one unidentified polar lipid. The major respiratory quinone was Q-7. Based on phenotypic, phylogenetic, chemotaxonomic and genomic results, we propose that strain SYSU DXS3258^T represents a novel species of a novel genus within the family Sphingobacteriaceae, for which the name Rubrolithibacter danxiaensis gen. nov., sp. nov. was proposed. The type strain is SYSU DXS3258^T (= MCCC 1K09420^T = KCTC 102322 ^T).