Age determination in domestic dogs using cementum annuli: Validity and methodology

IF 2.2 3区医学 Q1 MEDICINE, LEGAL

Forensic science international Pub Date : 2025-04-06 DOI:10.1016/j.forsciint.2025.112465

Yaren Çetin , Emine Keskin , Barış Batur , Ozan Gündemir , Özge Özgenç Çınar , Ahmet Ceylan , Caner Bakıcı

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引用次数: 0

Abstract

Age determination is an important part of forensic investigations and is used for assessment of population dynamics for animals and humans, understanding environmental conditions and so on. Age determinations using cementum annuli have been utilized for wild animals but have never been used for domestic dogs. The aim of this study was to test the accuracy of the method for domestic dogs and demonstrate the presence of cementum annuli. Ten domestic dogs were used for this experiment. The teeth were extracted from skulls via boiling and both mandibular and maxillary teeth were utilized. All teeth were decalcified using 10 % formic acid and 10 % formaldehyde solution. The decalcified teeth were embedded in paraffin and cut in 15 µm thickness. After staining with hematoxylin, the annuli were counted manually. The results obtained from this study suggest that domestic dogs indeed have cementum annuli and the annuli are countable, the number of annuli in teeth is compatible with the ages of animals, and canines of the same dogs show the same results meaning all canine teeth can be used for the age determination.

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用牙骨质环空测定家养狗的年龄：有效性和方法学

年龄测定是法医调查的重要组成部分，用于评估动物和人类的种群动态，了解环境条件等。利用牙骨质环空测定年龄已用于野生动物，但从未用于家养狗。本研究的目的是测试该方法对家养狗的准确性，并证明牙骨质环空的存在。实验采用10只家犬。用沸水法从颅骨中取出牙齿，同时使用下颌骨和上颌牙齿。用10% %甲酸和10% %甲醛溶液对所有牙齿进行脱钙。脱钙牙包埋于石蜡中，切成15 µm厚度。苏木精染色后，人工计数环空。本研究结果表明，家犬确实存在牙骨质环空，且牙骨质环空是可计数的，牙齿中牙骨质环空的数量与动物的年龄相适应，同一只犬的犬齿显示出相同的结果，这意味着所有犬齿都可以用于年龄测定。

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来源期刊

Forensic science international 医学-医学：法

CiteScore

5.00

自引率

9.10%

发文量

285

审稿时长

49 days

期刊介绍： Forensic Science International is the flagship journal in the prestigious Forensic Science International family, publishing the most innovative, cutting-edge, and influential contributions across the forensic sciences. Fields include: forensic pathology and histochemistry, chemistry, biochemistry and toxicology, biology, serology, odontology, psychiatry, anthropology, digital forensics, the physical sciences, firearms, and document examination, as well as investigations of value to public health in its broadest sense, and the important marginal area where science and medicine interact with the law. The journal publishes: Case Reports Commentaries Letters to the Editor Original Research Papers (Regular Papers) Rapid Communications Review Articles Technical Notes.