Inheritance and fitness costs of Cry1Ab and Cry1Ac resistance in Brazilian populations of Diatraea saccharalis (Lepidoptera: Crambidae)

IF 2.5 2区农林科学 Q1 AGRONOMY

Crop Protection Pub Date : 2025-03-24 DOI:10.1016/j.cropro.2025.107216

Juliana G. Rodrigues , Caroline Izabel R. Sakuno , Fabricio José B. Francischini , Karen Missy A. Komada , Fangneng Huang

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引用次数: 0

Abstract

The sugarcane borer, Diatraea saccharalis (F.), is the main insect pest on sugarcane in the Americas. Recently, transgenic Bacillus thuringiensis (Bt) sugarcane is planted in Brazil to control D. saccharalis. Resistance development is the greatest challenge for the sustainability of Bt crop biotechnology. Inheritance and fitness costs of resistance are essential information needed in developing effective insecticide resistance management (IRM) programs. In this study, multiple crossings were performed to characterize the inheritance and fitness costs of Cry1Ab and Cry1Ac resistance in two Brazilian populations (Cry1Ab_RR and Cry1Ac_RR) of D. saccharalis. Diet-incorporated and plant tissue bioassays showed that both the Cry1Ab and Cry1Ac resistance in the insect is inherited as a single, autosomal, and genetically incompletely recessive gene. Complementation tests showed that the Cry1Ab and Cry1Ac resistance in the two populations shared the same resistance gene. Leaf tissue bioassays suggested that the Cry1Ac sugarcane variety (CTC9001BT) tested in the study most likely meets the ‘high dose’ requirement for D. saccharalis as defined in the ‘high dose/refuge’ IRM strategy for Bt crops, while additional studies are needed to document the ‘high dose’ qualification for the tested Cry1Ab sugarcane variety (CTC20BT). Effective dominance levels of resistance decreased as Bt concentrations increased, suggesting that elevating Bt protein expressions in plants could be an effective strategy to make a ‘non-high dose’ plant become a ‘high dose’ plant. The Bt resistance in Cry1Ab_RR and Cry1Ac_RR was not associated with fitness costs on both non-Bt sugarcane and non-Bt corn/meridic diet. Knowledge generated from this study is useful in resistance risk assessment, monitoring, and development of IRM programs for the sustainable use of Bt sugarcane technology for pest management.

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来源期刊

Crop Protection 农林科学-农艺学

CiteScore

6.10

自引率

3.60%

发文量

200

审稿时长

29 days

期刊介绍： The Editors of Crop Protection especially welcome papers describing an interdisciplinary approach showing how different control strategies can be integrated into practical pest management programs, covering high and low input agricultural systems worldwide. Crop Protection particularly emphasizes the practical aspects of control in the field and for protected crops, and includes work which may lead in the near future to more effective control. The journal does not duplicate the many existing excellent biological science journals, which deal mainly with the more fundamental aspects of plant pathology, applied zoology and weed science. Crop Protection covers all practical aspects of pest, disease and weed control, including the following topics: -Abiotic damage- Agronomic control methods- Assessment of pest and disease damage- Molecular methods for the detection and assessment of pests and diseases- Biological control- Biorational pesticides- Control of animal pests of world crops- Control of diseases of crop plants caused by microorganisms- Control of weeds and integrated management- Economic considerations- Effects of plant growth regulators- Environmental benefits of reduced pesticide use- Environmental effects of pesticides- Epidemiology of pests and diseases in relation to control- GM Crops, and genetic engineering applications- Importance and control of postharvest crop losses- Integrated control- Interrelationships and compatibility among different control strategies- Invasive species as they relate to implications for crop protection- Pesticide application methods- Pest management- Phytobiomes for pest and disease control- Resistance management- Sampling and monitoring schemes for diseases, nematodes, pests and weeds.