Biochemical and cytogenetic consequences of settleable atmospheric particulate matter on Pacific white shrimp Litopenaeus vannamei

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology Pub Date : 2025-03-20 DOI:10.1016/j.aquatox.2025.107322

F. Copetti , C.R. Nobre , M.S. Paço , T.F.T. de Camargo , B.B. Moreno , M.N. Fernandes , R. Schveitzer , C.D.S. Pereira

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引用次数: 0

Abstract

This study hypothesizes that micro and nanoparticles of metals from metallurgical atmospheric emissions can trigger sublethal effects on the Pacific white shrimp Litopenaeus vannamei. We aimed to analyze cytotoxicity (Lysosomal Membrane Stability - LMS), Lipid peroxidation (LPO), genotoxicity (DNA strand break), and neurotoxicity (Acetylcholinesterase activity AChE) in shrimp exposed to environmentally relevant concentrations (0.001, 0.1, and 1.0 g.L⁻¹) of settleable atmospheric particulate matter (SePM) for different times (T2, T4, T7, T15 and T30 days), and in several tissues (gills, hemolymph, muscle,e and hepatopancreas). LPO within the first 2 day, and LMS showed significant differences. From the seventh to the fifteenth day of exposure, the concentration of 0.1 g.L⁻¹ exhibited significant effects. In the most extended exposure period (30 days), all concentrations triggered cytotoxicity effects on the Pacific white shrimp Litopenaeus vannamei. Thus, exposure to SePM can impair essential cellular functions, denoting a pre-pathological status. These findings underscore the potential hazards of metallurgical SePM to estuarine and farmed shrimp populations, emphasizing the need for ongoing monitoring and effective mitigation strategies to ensure aquaculture sustainability.

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可沉降大气颗粒物对凡纳滨对虾的生化和细胞遗传学影响

本研究假设来自冶金大气排放的金属微粒和纳米粒子可对凡纳滨对虾（Litopenaeus vannamei）产生亚致死效应。我们的目的是分析细胞毒性（溶酶体膜稳定性- LMS）、脂质过氧化（LPO）、遗传毒性（DNA链断裂）和神经毒性（乙酰胆碱酯酶活性AChE）在不同时间（T2、T4、T7、T15和T30天）暴露于环境相关浓度（0.001、0.1和1.0 g.L−1）的对虾，以及几种组织（鳃、血淋巴、肌肉、e和肝胰腺）。LPO在前2天内，与LMS有显著差异。暴露第7天至第15天，浓度为0.1 g。L−1表现出显著的影响。在最长暴露期（30 d），所有浓度均对凡纳滨对虾产生细胞毒性作用。因此，暴露于SePM可以损害基本的细胞功能，表明病理前状态。这些发现强调了冶金SePM对河口和养殖对虾种群的潜在危害，强调了持续监测和有效缓解战略的必要性，以确保水产养殖的可持续性。

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来源期刊

Aquatic Toxicology 环境科学-毒理学

CiteScore

7.10

自引率

4.40%

发文量

250

审稿时长

56 days

期刊介绍： Aquatic Toxicology publishes significant contributions that increase the understanding of the impact of harmful substances (including natural and synthetic chemicals) on aquatic organisms and ecosystems. Aquatic Toxicology considers both laboratory and field studies with a focus on marine/ freshwater environments. We strive to attract high quality original scientific papers, critical reviews and expert opinion papers in the following areas: Effects of harmful substances on molecular, cellular, sub-organismal, organismal, population, community, and ecosystem level; Toxic Mechanisms; Genetic disturbances, transgenerational effects, behavioral and adaptive responses; Impacts of harmful substances on structure, function of and services provided by aquatic ecosystems; Mixture toxicity assessment; Statistical approaches to predict exposure to and hazards of contaminants The journal also considers manuscripts in other areas, such as the development of innovative concepts, approaches, and methodologies, which promote the wider application of toxicological datasets to the protection of aquatic environments and inform ecological risk assessments and decision making by relevant authorities.