Polydopamine Powered Droplet Electricity Generator for Protein Assay with CRISPR/Cas Enabled Amplification

IF 9.6 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Nano Letters Pub Date : 2025-04-07 DOI:10.1021/acs.nanolett.5c00877

Xiaolin Qu, Yuyan Yue, Yuguo Tang, Jin Jiao, Peng Miao

引用次数: 0

Abstract

Protein function investigation and clinical assay are fundamental to modern biology and medical diagnostics. Flap endonuclease 1 (FEN1), a key enzyme in DNA replication and repair, plays a critical role in the progression of many diseases. Taking FEN1 as an example, we present a novel protein detection platform combining triboelectric nanogenerator (TENG) and CRISPR/Cas technologies. As a specific form of TENG, the transistor-droplet electricity generator (TDEG) is explored, which offers a low-cost, simple fabrication approach with real-time detection capability. Meanwhile, the FEN1 activated CRISPR/Cas system catalyzes the reactions on the three-dimensional DNA tetrahedron interface, promising the high sensitivity. This work not only demonstrates a powerful method for rapid protein detection but also pioneers the integration of CRISPR/Cas with TENG. It has a great prospect for future development of TENG sensors.

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聚多巴胺供电液滴发生器，用于蛋白质测定和 CRISPR/Cas 扩增

蛋白质功能研究和临床检测是现代生物学和医学诊断的基础。皮瓣内切酶1 （Flap endonucase 1, FEN1）是DNA复制和修复的关键酶，在许多疾病的进展中起着关键作用。以FEN1为例，我们提出了一种结合摩擦电纳米发电机（TENG）和CRISPR/Cas技术的新型蛋白检测平台。作为TENG的一种特殊形式，我们探索了晶体管液滴发电机（TDEG），它提供了一种低成本、简单的制造方法，并具有实时检测能力。同时，FEN1激活的CRISPR/Cas系统在三维DNA四面体界面上催化反应，具有较高的灵敏度。这项工作不仅展示了一种强大的快速蛋白质检测方法，而且开创了CRISPR/Cas与TENG结合的先驱。它对未来TENG传感器的发展具有广阔的前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nano Letters 工程技术-材料科学：综合

CiteScore

16.80

自引率

2.80%

发文量

1182

审稿时长

1.4 months

期刊介绍： Nano Letters serves as a dynamic platform for promptly disseminating original results in fundamental, applied, and emerging research across all facets of nanoscience and nanotechnology. A pivotal criterion for inclusion within Nano Letters is the convergence of at least two different areas or disciplines, ensuring a rich interdisciplinary scope. The journal is dedicated to fostering exploration in diverse areas, including: - Experimental and theoretical findings on physical, chemical, and biological phenomena at the nanoscale - Synthesis, characterization, and processing of organic, inorganic, polymer, and hybrid nanomaterials through physical, chemical, and biological methodologies - Modeling and simulation of synthetic, assembly, and interaction processes - Realization of integrated nanostructures and nano-engineered devices exhibiting advanced performance - Applications of nanoscale materials in living and environmental systems Nano Letters is committed to advancing and showcasing groundbreaking research that intersects various domains, fostering innovation and collaboration in the ever-evolving field of nanoscience and nanotechnology.