Urobiome and Inflammation: A Systematic Review on Microbial Imbalances and Diagnostic Tools for Urinary Disorders

IF 2.1 3区医学 Q2 UROLOGY & NEPHROLOGY

Urology Pub Date : 2025-06-01 DOI:10.1016/j.urology.2025.03.050

Sara Palumbo , Giuseppe Lucarelli , Francesco Lasorsa , Rocco Damiano , Riccardo Autorino , Achille Aveta , Gianluca Spena , Sisto Perdonà , Pierluigi Russo , Carlo Giulioni , Angelo Cafarelli , Marco Finati , Salvatore Siracusano , Savio Domenico Pandolfo

{"title":"Urobiome and Inflammation: A Systematic Review on Microbial Imbalances and Diagnostic Tools for Urinary Disorders","authors":"Sara Palumbo , Giuseppe Lucarelli , Francesco Lasorsa , Rocco Damiano , Riccardo Autorino , Achille Aveta , Gianluca Spena , Sisto Perdonà , Pierluigi Russo , Carlo Giulioni , Angelo Cafarelli , Marco Finati , Salvatore Siracusano , Savio Domenico Pandolfo","doi":"10.1016/j.urology.2025.03.050","DOIUrl":null,"url":null,"abstract":"<div><h3>OBJECTIVE</h3><div>To synthesize current knowledge on urobiome alterations, innovative diagnostic advancements, and emerging therapeutic strategies targeting urobiome dysbiosis in inflammatory urinary tract disorders, including urinary tract infections, nongonococcal urethritis, and interstitial cystitis.</div></div><div><h3>METHODS</h3><div>A systematic review was conducted by screening the most important scientific databases. The search included the keywords: (microbiome) OR (microbial) OR (bacteria) OR (bacterial profile) AND (urine) OR (urinary) AND (first-morning sample) OR (first void). Only original studies in English involving human specimens were considered.</div></div><div><h3>RESULTS</h3><div>Of the 760 articles initially identified, a final sample of 20 original studies met the inclusion criteria. Disruptions in the urobiome composition were associated with increased colonization by pathogens such as <em>Escherichia coli</em> and <em>Mycoplasma genitalium</em>, resulting in inflammation and recurrent urinary conditions. Advanced diagnostic techniques, including metaproteomics, metagenomics, and point-of-care assays such as <em>Neisseria gonorrhoeae</em> lateral flow assay, demonstrated enhanced capabilities for rapid pathogen detection and differentiation of inflammatory conditions. Therapeutic interventions targeting urobiome dysbiosis, particularly probiotics (<em>Lactobacillus rhamnosus</em>, <em>L. reuteri</em>, <em>L. crispatus</em>), showed promising efficacy in reducing recurrence and inflammation in clinical trials.</div></div><div><h3>CONCLUSION</h3><div>Urobiome dysbiosis plays a critical role in inflammatory urinary tract disorders. Innovative diagnostic methods and targeted therapeutic approaches, especially probiotics, offer substantial potential to improve patient outcomes. Further research is warranted to refine these strategies and validate their clinical applicability.</div></div>","PeriodicalId":23415,"journal":{"name":"Urology","volume":"200 ","pages":"Pages 206-215"},"PeriodicalIF":2.1000,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Urology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S009042952500305X","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"UROLOGY & NEPHROLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

OBJECTIVE

To synthesize current knowledge on urobiome alterations, innovative diagnostic advancements, and emerging therapeutic strategies targeting urobiome dysbiosis in inflammatory urinary tract disorders, including urinary tract infections, nongonococcal urethritis, and interstitial cystitis.

METHODS

A systematic review was conducted by screening the most important scientific databases. The search included the keywords: (microbiome) OR (microbial) OR (bacteria) OR (bacterial profile) AND (urine) OR (urinary) AND (first-morning sample) OR (first void). Only original studies in English involving human specimens were considered.

RESULTS

Of the 760 articles initially identified, a final sample of 20 original studies met the inclusion criteria. Disruptions in the urobiome composition were associated with increased colonization by pathogens such as Escherichia coli and Mycoplasma genitalium, resulting in inflammation and recurrent urinary conditions. Advanced diagnostic techniques, including metaproteomics, metagenomics, and point-of-care assays such as Neisseria gonorrhoeae lateral flow assay, demonstrated enhanced capabilities for rapid pathogen detection and differentiation of inflammatory conditions. Therapeutic interventions targeting urobiome dysbiosis, particularly probiotics (Lactobacillus rhamnosus, L. reuteri, L. crispatus), showed promising efficacy in reducing recurrence and inflammation in clinical trials.

CONCLUSION

Urobiome dysbiosis plays a critical role in inflammatory urinary tract disorders. Innovative diagnostic methods and targeted therapeutic approaches, especially probiotics, offer substantial potential to improve patient outcomes. Further research is warranted to refine these strategies and validate their clinical applicability.

查看原文本刊更多论文

泌尿组和炎症：泌尿系统疾病的微生物失衡和诊断工具的系统综述。

目的：综合目前关于尿路菌群改变的知识，创新的诊断进展，以及针对炎性尿路疾病（包括尿路感染（uti），非淋球菌性尿道炎（NGU）和间质性膀胱炎）尿路菌群失调的新治疗策略。方法：通过筛选最重要的科学数据库进行系统评价。搜索的关键词包括：（微生物组）或（微生物）或（细菌）或（细菌谱）和（尿液）或（尿液）和（第一个早晨的样本）或（第一个空洞）。只考虑涉及人类标本的英文原创研究。结果：在最初确定的760篇文章中，20篇原始研究的最终样本符合纳入标准。尿组组成的破坏与大肠杆菌和生殖支原体等病原体的定植增加有关，导致炎症和复发性泌尿系统疾病。先进的诊断技术，包括宏蛋白质组学、宏基因组学和NG-LFA等即时检测技术，显示出快速检测病原体和区分炎症状况的能力增强。针对尿生态失调的治疗干预，特别是益生菌（鼠李糖乳杆菌、罗伊氏乳杆菌、crispatus乳杆菌），在临床试验中显示出减少复发和炎症的良好疗效。结论：尿组失调在炎症性尿路疾病中起关键作用。创新的诊断方法和有针对性的治疗方法，特别是益生菌，为改善患者的预后提供了巨大的潜力。需要进一步的研究来完善这些策略并验证其临床适用性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Urology 医学-泌尿学与肾脏学

CiteScore

3.30

自引率

9.50%

发文量

716

审稿时长

59 days

期刊介绍： Urology is a monthly, peer–reviewed journal primarily for urologists, residents, interns, nephrologists, and other specialists interested in urology The mission of Urology®, the "Gold Journal," is to provide practical, timely, and relevant clinical and basic science information to physicians and researchers practicing the art of urology worldwide. Urology® publishes original articles relating to adult and pediatric clinical urology as well as to clinical and basic science research. Topics in Urology® include pediatrics, surgical oncology, radiology, pathology, erectile dysfunction, infertility, incontinence, transplantation, endourology, andrology, female urology, reconstructive surgery, and medical oncology, as well as relevant basic science issues. Special features include rapid communication of important timely issues, surgeon''s workshops, interesting case reports, surgical techniques, clinical and basic science review articles, guest editorials, letters to the editor, book reviews, and historical articles in urology.