Serovar distribution, virulence gene and antibiotic susceptibility profiling of Salmonella enterica isolated from seafood in Mumbai, India

IF 3.3 3区医学 Q3 IMMUNOLOGY

Microbial pathogenesis Pub Date : 2025-04-03 DOI:10.1016/j.micpath.2025.107530

Parmanand Prabhakar , Manjusha Lekshmi , Parvathi Ammini , Binaya Bhusan Nayak , Sanath Kumar

{"title":"Serovar distribution, virulence gene and antibiotic susceptibility profiling of Salmonella enterica isolated from seafood in Mumbai, India","authors":"Parmanand Prabhakar , Manjusha Lekshmi , Parvathi Ammini , Binaya Bhusan Nayak , Sanath Kumar","doi":"10.1016/j.micpath.2025.107530","DOIUrl":null,"url":null,"abstract":"<div><div>Salmonella contamination of seafood and its environment is an important public health concern. In this study, Salmonella enterica isolated from seafood in Mumbai, India were seroptyped and further characterized with respect to the distribution of virulence genes associated with Salmonella Pathogenicity Islands (SPIs) and their antimicrobial susceptibilities. The study included 89 isolates which were screened for 11 genes associated with five pathogenicity islands (SPI-1 to SPI-5), two phage-encoded virulence genes, and an enterotoxin gene. The isolates were serotyped into 10 serovars namely S. Typhimurium, S. Bareilly, S. Weltevreden, S. Volta, S. Newport, S. Tennessee, S. Kentucky, S. Lindenburg, S. Paratyphi B and S. Alachua. S. Bareilly (33) was the predominant serovar, followed by S. Typhimurium (15), S. Newport (10), S. Kentucky (9), S. Paratyphi B (8), and S. Tennessee (7). Ten S. Typhimurium and one each of S. Kentucky and S. Paratyphi B isolates possessed all the virulence genes tested, while the remaining isolates lacked one or more virulence genes. The antibiotic susceptibility testing revealed that the isolates were resistant against third-generation cephalosporins, quinolones, fluoroquinolones and tetracycline antibiotics, but sensitive to ceftazidime, aztreonam, carbapenems, chloramphenicol, and aminoglycosides. The diverse serovars of Salmonella contaminating fresh seafood in this geographical region constitute a notable public health concern.</div></div>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":"204 ","pages":"Article 107530"},"PeriodicalIF":3.3000,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microbial pathogenesis","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0882401025002554","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"IMMUNOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Salmonella contamination of seafood and its environment is an important public health concern. In this study, Salmonella enterica isolated from seafood in Mumbai, India were seroptyped and further characterized with respect to the distribution of virulence genes associated with Salmonella Pathogenicity Islands (SPIs) and their antimicrobial susceptibilities. The study included 89 isolates which were screened for 11 genes associated with five pathogenicity islands (SPI-1 to SPI-5), two phage-encoded virulence genes, and an enterotoxin gene. The isolates were serotyped into 10 serovars namely S. Typhimurium, S. Bareilly, S. Weltevreden, S. Volta, S. Newport, S. Tennessee, S. Kentucky, S. Lindenburg, S. Paratyphi B and S. Alachua. S. Bareilly (33) was the predominant serovar, followed by S. Typhimurium (15), S. Newport (10), S. Kentucky (9), S. Paratyphi B (8), and S. Tennessee (7). Ten S. Typhimurium and one each of S. Kentucky and S. Paratyphi B isolates possessed all the virulence genes tested, while the remaining isolates lacked one or more virulence genes. The antibiotic susceptibility testing revealed that the isolates were resistant against third-generation cephalosporins, quinolones, fluoroquinolones and tetracycline antibiotics, but sensitive to ceftazidime, aztreonam, carbapenems, chloramphenicol, and aminoglycosides. The diverse serovars of Salmonella contaminating fresh seafood in this geographical region constitute a notable public health concern.

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Microbial pathogenesis 医学-免疫学

CiteScore

7.40

自引率

2.60%

发文量

472

审稿时长

56 days

期刊介绍： Microbial Pathogenesis publishes original contributions and reviews about the molecular and cellular mechanisms of infectious diseases. It covers microbiology, host-pathogen interaction and immunology related to infectious agents, including bacteria, fungi, viruses and protozoa. It also accepts papers in the field of clinical microbiology, with the exception of case reports. Research Areas Include: -Pathogenesis -Virulence factors -Host susceptibility or resistance -Immune mechanisms -Identification, cloning and sequencing of relevant genes -Genetic studies -Viruses, prokaryotic organisms and protozoa -Microbiota -Systems biology related to infectious diseases -Targets for vaccine design (pre-clinical studies)